EMAGE mouse gene expression spatial database

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EMAGE:3706

Rhob ras homolog gene family, member B ( MGI:107949)
TS15 (9.5 dpc)
in situ hybridisation

Data Images


Figure 4A. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00333-6] Mech Dev 95: 211-4, Henderson DJ; Ybot-Gonzalez P; Copp AJ, RhoB is expressed in migrating neural crest and endocardial cushions of the developing mouse embryo Copyright 2000.	Figure 4B. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00333-6] Mech Dev 95: 211-4, Henderson DJ; Ybot-Gonzalez P; Copp AJ, RhoB is expressed in migrating neural crest and endocardial cushions of the developing mouse embryo Copyright 2000.

Expression pattern clarity:
Find spatially similar expression patterns:

Notes:

Note: Transverse sections. a) Arrowheads point to right ventricle. b) Arrowheads point to outflow tract and aortic sac endocardium. a, atria; as, aortic sac; avc, atrioventricular cushion tissue; nt, neural tube; v, ventricle. Scale bar, 175 microns.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

3D mapping

spatial mapping

View mapped 3D expression image

Download individual expression domains:

3706_voxel_moderate_3D_1.wlz

3706_voxel_weak_3D_1.wlz

3706_voxel_notDetected_3D_1.wlz

3706_voxel_strong_3D_1.wlz

3706_voxel_moderate_3D_2.wlz

3706_voxel_weak_3D_2.wlz

3706_voxel_notDetected_3D_2.wlz

3706_voxel_strong_3D_2.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3706_all_domains.zip

Find spatially similar expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

atrioventricular canal

detected

outflow tract

detected

regional

Expression in the endocardium of the proximal outflow tract. Expression in outflow tract and aortic sac endocardium.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:1862033
Entity Detected:	Rhob, ras homolog gene family, member B ( MGI:107949)
Sequence:	sense strand is shown >MGI:1862033 CGTGTTCGAGAACTATGTGGCGGACATCGAGGTGGACGGCAAGCAGGTGGAGCTGGCGCTGTGGGACACG GCAGGCCAGGAGGACTACGATCGTTTACGGCCGCTCTCCTATCCGGACACCGACGTCATCCTTATGTGCT TCTCGGTGGACAGCCCGGACTCTCTCGAGAACATCCCCGAGAAGTGGGTGCCCGAGGTAAAGCACTTCTG CCCCAATGTGCCCATCATCCTGGTGGCCAACAAAAAAGACCTGCGCAGCGACGAGCATGTCCGCACGGAG CTGGCCCGCATGAAGCAGGAGCCAGTGCGCACGGATGACGGCCGCGCCATGGCGGTGCGCATCCAAGCCT ATGACTACCTCGAGTGCTCGGCCAAGACCAAGGAGGGCGTGCGCGAGGTTTTTGAGACGGCCACGCGCGC CGCGCTGCAGAAGCGCTACGGATCCCAGAATGGCTGCATCAACT
	nt 4063 - nt 4526 of X99963.1
Notes:	The Rhob probe used in this study by Henderson et al., 2000 [PMID:10906464] "was obtained from GenBank [sequence] (accession number x99963) and a 463 bp fragment was amplified by RT-PCR. This corresponds to the region between positions 4063 and 4526 of the cDNA sequence".
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD-1
Age:	9.5 dpc
Theiler Stage:	TS15
Mutations:	none (wild-type)
Preparation:	sectioned wholemount

Procedures

Fixation:

4% paraformaldehyde

General Information

Authors:	Henderson DJ; Ybot-Gonzalez P; Copp AJ, 2000 [PMID:10906464] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/S0925-4773(00)00333-6] [ PMID:10906464] Henderson DJ, Ybot-Gonzalez P, Copp AJ 2000 RhoB is expressed in migrating neural crest and endocardial cushions of the developing mouse embryo. Mech Dev (95):211-4
Links:	MGI:2384277 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source