EMAGE mouse gene expression spatial database

EMAGE:3708

Slc8a1 solute carrier family 8 (sodium/calcium exchanger), member 1 ( MGI:107956)
TS13 (9 Somite no.)
in situ hybridisation

Data Images


Figure 2D. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(99)00177-X] Mech Dev 88: 119-22, Koushik SV; Bundy J; Conway SJ, Sodium-calcium exchanger is initially expressed in a heart-restricted pattern within the early mouse embryo Copyright 1999.	Figure 2D'. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(99)00177-X] Mech Dev 88: 119-22, Koushik SV; Bundy J; Conway SJ, Sodium-calcium exchanger is initially expressed in a heart-restricted pattern within the early mouse embryo Copyright 1999.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Arrows: NCX1 is expressed along the entire heart tube and sinus venosa.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

bulbus cordis

detected

common atrial chamber

detected

primitive ventricle

detected

outflow tract

detected

sinus venosus

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:2389076
Entity Detected:	Slc8a1, solute carrier family 8 (sodium/calcium exchanger), member 1 ( MGI:107956)
Sequence:	sense strand is shown >MGI:2389076 AAGAAACCGAATGGAGAGACCACCAAGACGACGGTGAGAATCTGGAACGAGACTGTGTCGAACCTGACCT TGATGGCCCTGGGATCTTCTGCTCCTGAGATTCTCCTGTCAGTCATTGAAGTGTGCGGCCATAACTTCAC CGCAGGGGACCTGGGTCCCAGCAGGATCGTGGGAAGTGCTGCCTTTAACATGTTCATCATAATCGCACTC TGTGTTTACGTGGTCCCTGATGGAGAGACAAGGAAGATCAAGCATCTGCGTGTGTTCTTTGTGACAGCAG CCTGGAGCATCTTTGCCTATACCTGGCTTTATATAATCTTGTCTGTCAGCTCTCCTGGAGTTGTGGAGGT CTGGGAAGGCTTGCTTACTTTCTTCTTCTTTCCCATCTGCGTTGTGTTCGCGTGGGTAGCAGACAGGCGG CTTCTCTTTTACAAGTATGTCTACAAGCGGTACAGGGCCGGCAAGCAGAGGGGGATGATCATTGAACATG AAGGAGACAGACCAGCTTCCAAAACTGAAATCGAAATGGATGGGAAAGTGGTCAACTCTCATGTTGACAA TTTCTTAGATGGGGCTCTGGTTTTGGAAGTTGATGAGAGGGACCA
	nt 442 - nt 1046 of AF004666.1
Notes:	The Slc8a1 (NCX1) probe used in this study by Koushik et al., 1999 [PMID:10525196] was "generated by PCR amplification, using primers based on GenBank mRNA sequence (AF004666). NCX1-specific primers were designed to amplify the region between base pairs 442-461 (upstream primer 5'-AAGAAACCGAATGGAGAGAC-3') and 1027-1046 within exon 2 (downstream primer 5'-TGGTCCCTCTCATCAACTTC-3')".
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

General Information

Authors:	Koushik SV; Bundy J; Conway SJ, 1999 [PMID:10525196] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/S0925-4773(99)00177-X] [ PMID:10525196] Koushik SV, Bundy J, Conway SJ 1999 Sodium-calcium exchanger is initially expressed in a heart-restricted pattern within the early mouse embryo. Mech Dev (88):119-22
Links:	MGI:2429570 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source