EMAGE mouse gene expression spatial database

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EMAGE:3724

En1 engrailed 1 ( MGI:95389)
TS12 (6-8 Somite no.)
in situ hybridisation

Data Images


Fig 4BCopyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1996.0120] Dev Biol 175: 347-57, Avantaggiato V; Acampora D; Tuorto F; Simeone A, Retinoic acid induces stage-specific repatterning of the rostral central nervous system. Copyright 1996	Fig 4ACopyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1996.0120] Dev Biol 175: 347-57, Avantaggiato V; Acampora D; Tuorto F; Simeone A, Retinoic acid induces stage-specific repatterning of the rostral central nervous system. Copyright 1996

Expression pattern clarity:
Find spatially similar expression patterns:

Notes:

Figure 4A' is a brightfield representation of the section shown in Figure 4B. Image annotations: Arrow points to the position corresponding to the Pax-2 posterior border; fb - forebrain; mb - midbrain; hb - hindbrain; fg - foregut.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

3D mapping

spatial mapping

View mapped 3D expression image

Download individual expression domains:

3724_voxel_strong_3D_1.wlz

3724_voxel_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3724_all_domains.zip

Find spatially similar expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

future midbrain

detected

regional

The En-1 expression domain includes the the rostral-most hindbrain and posterior two-thirds of the mesencephalon, partially overlapping the Wnt-1 domain.

future hindbrain

detected

regional

The En-1 expression domain includes the the rostral-most hindbrain and posterior two-thirds of the mesencephalon, partially overlapping the Wnt-1 domain.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:1339351
Entity Detected:	En1, engrailed 1 ( MGI:95389)
Sequence:	sense strand is shown >MGI:1339351 CAGCAGCCGGAGCCTAAAAGTCAGCGCGACTCGGGCCTCGGCGCGGTGGCAGCGGCGGCCCCGAGCGGCC TCAGTCTGAGTCTGAGCCCAGGAGCCAGCGGCAGCAGCGGCAGCGATGGAGACAGCGTGCCGGTGTCCCC GCAGCCAGCGCCCCCGTCGCCTCCTGCGGCACCCTGTCTGCCGCCCCTGGCCCATCACCCGCACCTCCCC CCGCATCCCCCGCCCCCGCCGCCGCCGCCGCCGCCGCCACCGCAGCATCTCGCGGCGCCTGCTCACCAGC CGCAGCCCGCGGCCCAGCTGCACCGCACCACCAACTTTTTCATCGATAACATCCTAAGGCCCGATTTCGG TTGCAAAAAGGAACAGCCCCTGCCTCAGCTCCTGGTGGCTTCGGCTGCAGCCGGAGGAGGCGCAGCAGCA GGAGGAGGAAGCCGCGTGGAGCGTGACCGAGGCCAGACTGGTGCAGGTAGAGACCCCGTTCACTCTCTGG GCACACGAGCTTCGGGGGCTGCCTCGCTCTTGTGTGCTCCAGATGCGAACT
	nt 362 - nt 902 of NM_010133.1
Notes:	The En1 probe used in this study by Avantaggiato et al., 1996 [PMID:8626038] is described as a PCR amplified probe spanning the region between amino acids 3 and 183. Editor note: The coordinates with respect to the GenBank En1 cDNA RefSeq NM_010133.1 above were deduced from this information.
Chemistry:	RNA
Strand:	antisense
Label:	S35

Specimen

Organism:	mouse
Age:	6-8 Somite no.
Theiler Stage:	TS12
Mutations:	none (wild-type)
Preparation:	section

Procedures

Staining procedure:

autoradiography

General Information

Authors:	Avantaggiato V; Acampora D; Tuorto F; Simeone A, 1996 [PMID:8626038] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1006/dbio.1996.0120] [ PMID:8626038] Avantaggiato V, Acampora D, Tuorto F, Simeone A 1996 Retinoic acid induces stage-specific repatterning of the rostral central nervous system. Dev Biol (175):347-57
Links:	MGI:1339352 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source