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EMAGE:3759

Jag2 jagged 2 ( MGI:1098270)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3759
Figure 1B. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(97)00146-9] Mech Dev 69: 203-7, Valsecchi C; Ghezzi C; Ballabio A; Rugarli EI, JAGGED2: a putative Notch ligand expressed in the apical ectodermal ridge and in sites of epithelial-mesenchymal interactions Copyright 1997.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: mx, maxillary component of the first branchial arch; md, mandibular component of the first branchial arch; np, nasal process; II, second branchical arch. Arrowheads point to expression in the apical ecodermal ridges.
Expression Pattern Description
Spatial Annotation:
EMAGE:3759Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3759_wholemount_strong_3D_1.wlz
3759_wholemount_moderate_3D_1.wlz
3759_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3759_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
1st branchial groove ectoderm
detected detected
1st branchial arch maxillary component ectoderm
detected detected
1st branchial arch mandibular component ectoderm
detected detected
1st branchial arch maxillary-mandibular groove ectoderm
detected detected
2nd branchial groove ectoderm
detected detected
2nd branchial membrane ectoderm
detected detected
3rd branchial arch ectoderm
detected detected
forelimb bud apical ectodermal ridge
detected detected
hindlimb bud apical ectodermal ridge
detected detected
latero-nasal process ectoderm
detected detected
medial-nasal process ectoderm
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2429866
Entity Detected:Jag2, jagged 2 ( MGI:1098270)
Sequence:sense strand is shown

>MGI:2429866
CAATGCTGAGCCTGACCAATACCTCTGCGCCTGCCCAGATGGCTACTTGGGCAAGAACTGTGAGCGGGCT
GAGCACGCCTGTGCCTCCAACCCGTGTGCCAATGGGGGCTCTTGCCACGAAGTGCCATCTGGCTTTGAAT
GCCACTGTCCGTCAGGATGGAGCGGACCCACCTGTGCGCTCGACATTGATGAGTGTGCCTCTAACCCATG
TGCAGCGGGTGGTACCTGCGTGGATCAGGTGGACGGCTTCGAGTGCATCTGCCCGGAGCAGTGGGTGGGG
GCTACTTGCCAGCTGGACGCCAATGAGTGTGAAGGGAAGCCGTGCCTTAATGCTTTTTCTTGCAAAAACC
TGATTGGCGGCTATTACTGTGATTGCCTCCCGGGCTGGAAGGGCATCAACTGCCAAATCAACATCAACGA
TTGTCATGGGCAGTGTCAGCATGGGGGCACCTGCAAGGACCTGGTCAATGGGTACCAGTGTGTGTGCCCG
CGGGGCTTTGGAGGTCGCCATTGCGAACTAGAGTACGACAAGTGTGCCAGCAGCCCCTGCCGCCGGGGTG
GCATCTGCGAGGACCTGGTGGATGGCTTCCGCTGCCACTGCCCACGGGGCCTCTCTGGGCTGCACTGTGA
GGTGGACATGGATCTCTGTGAACCAAGCCCCTGCCTCAACGGTGCTCGCTGCTACAACCTTGAGGGTGAC
TACTACTGCGCCTGCCCAGAAGACTTTGGTGGCAAGAACTGCTCAGTGCCCAGGGACACATGCCCTGGCG
GGGCATGTAGAGTGATCGATGGCTGCGGGTTCGAGGCAGGGTCCAGGGCACGCGGTGTCGCACCCTCTGG
TATATGTGGCCCTCACGGGCACTGCGTTAGCCTGCCTGGGGGAAACTTCTCCTGCATCTGTGACAGCGGC
TTCACAGGCACCTACTGCCATGAAAACATTGACGACTGCATGGGCCAGCCCTGCCGCAACGGGGGCACGT
GCATTGACGAAGTGGACTCCTTCCGCTGCTTCTGCCCCAGTGGCTGGGAAGGAGAACTCTGTGACATCAA
TCCCAACGACTGCCTCCCCGACCCCTGCCACAGCCGCGGCCGCTGCTATGACCTGGTCAATGACTTCTAC
TGTGCCTGTGACGATGGCTGGAAGGGCAAGACCTGCCACTCACGCGAGTTCCAGTGTGACGCCTACACCT
GCAGCAACGGTGGCACATGCTATGACAGCGGCGACACCTTTCCTGCGCGTCCTCCGGGCTGGAAGGGCAG
CACCTGCACCATCGCCAAGAACAGCAGCTGTGTGCCCAATCCC
nt 1 - nt 1303 of Y14495.1
Notes:The Jag2 probe used in this study by Valsecchi et al., 1997 [PMID:9486542] is described as such: "Clone S2 (GenBank Y14495) was used as template for the digoxygenin-labelled RNA probes".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Valsecchi C; Ghezzi C; Ballabio A; Rugarli EI, 1997 [PMID:9486542] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(97)00146-9] [ PMID:9486542] Valsecchi C, Ghezzi C, Ballabio A, Rugarli EI 1997 JAGGED2: a putative Notch ligand expressed in the apical ectodermal ridge and in sites of epithelial-mesenchymal interactions. Mech Dev (69):203-7
Links:MGI:2429962 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI