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EMAGE:3785

Irx3 Iroquois related homeobox 3 (Drosophila) ( MGI:1197522)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:3785
Fig2B.Copyright: Reprinted with permission from Elsevier from [ [doi:10.1016/S0925-4773(99)00263-4] Mech Dev 91: 317-21, Cohen DR; Cheng CW; Cheng SH; Hui C, Expression of two novel mouse iroquois homeobox genes during neurogenesis Copyright 2000

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: cm, cephalic mesenchyme; fg, foregut; fl, forelimb; hl, hindlimb.
Expression Pattern Description
Spatial Annotation:
EMAGE:3785Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3785_wholemount_strong_3D_1.wlz
3785_wholemount_moderate_3D_1.wlz
3785_wholemount_weak_3D_1.wlz
3785_wholemount_possible_3D_1.wlz
3785_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3785_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future midbrain
detected detected
regionalExpression is throughout the dorsolateral region. Absent at the mid/hindbrain boundary.
future hindbrain
detected detected
regionalExpression is in the ventrolateral region of the metencephalon, absent at the mid/hindbrain boundary.
neural tube lateral wall
detected detected
regionalExpression is in the ventricular layer.
forelimb bud
detected detected
hindlimb ridge
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:26774280
Entity Detected:Irx3, Iroquois related homeobox 3 (Drosophila) ( MGI:1197522)
Notes:The Irx3 probe used in this study by Cohen et al, 2000 [PMID:10704856] is described as follows "Digoxigenin-labeled RNA probes were synthesized from either of the following templates: pIrx3a, a 645 bp EcoRI-BamHI fragment containing mostly the 3'UTR of Irx3 (MGI:26774280); pIrx3b, a 1.3 kb EcoRI-BamHI fragment containing the ORF of Irx3 (MGI:2677429)". Editors note: it is not specified which of these probes was actually used in this particular assay, however the authors say that both probes yield similar patterns.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Cohen DR; Cheng CW; Cheng SH; Hui C, 2000 [PMID:10704856] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(99)00263-4] [ PMID:10704856] Cohen DR, Cheng CW, Cheng SH, Hui CC 2000 Expression of two novel mouse Iroquois homeobox genes during neurogenesis. Mech Dev (91):317-21
Links:MGI:2677865 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI