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EMAGE:3787

Irx2 Iroquois related homeobox 2 (Drosophila) ( MGI:1197526)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:3787
Fig2D.Copyright: Reprinted with permission from Elsevier from [ [ [doi:10.1016/S0925-4773(99)00263-4] Mech Dev 91: 317-21, Cohen DR; Cheng CW; Cheng SH; Hui C, Expression of two novel mouse iroquois homeobox genes during neurogenesis Copyright 2000

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: ba, branchial arch; fg, foregut.
Expression Pattern Description
Spatial Annotation:
EMAGE:3787Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3787_wholemount_strong_3D_1.wlz
3787_wholemount_moderate_3D_1.wlz
3787_wholemount_weak_3D_1.wlz
3787_wholemount_possible_3D_1.wlz
3787_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3787_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future midbrain
detected detected
regionalExpression is in a dorsal domain in the mesencephalon. Absent at the mid/hindbrain boundary.
future hindbrain
detected detected
regionalExpression is in a dorsal domain in the mesencephalon. Absent at the mid/hindbrain boundary.
future spinal cord
weak weak
branchial arch
detected detected
Expression is in the epithelial components.
foregut
detected detected
Expression is in the epithelial components.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2677434
Entity Detected:Irx2, Iroquois related homeobox 2 (Drosophila) ( MGI:1197526)
Notes:NOTE: The authors refer to this gene as Irx6 however BLAST sequence comparison of the 'Irx6' sequence AF165986 mentioned in this paper against GenBank confirms that this gene is Irx2 and not Irx6. The Irx2 probe used in this study by Cohen et al, 2000 [PMID:10704856] is described as being transcribed from "pIrx6, a 1.3 kb SalI fragment containing part of the ORF and the 3'UTR of Irx6 (i.e. Irx2)". Editors Note: Restriction digestion of numerous Irx2 cDNA database sequences with SalI identifies a single site within the ORF. It is therefore assumed that the other SalI site is derived from linker/MCS sequence at the 3' end of the clone. Despite this, a 1.3kb fragment cannot be identifed from database searching and larger fragments are found (e.g. 1.7kb in NM_010574.2). The specific sequence of this probe is unclear from available information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Cohen DR; Cheng CW; Cheng SH; Hui C, 2000 [PMID:10704856] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(99)00263-4] [ PMID:10704856] Cohen DR, Cheng CW, Cheng SH, Hui CC 2000 Expression of two novel mouse Iroquois homeobox genes during neurogenesis. Mech Dev (91):317-21
Links:MGI:2677867 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI