| Type: | in situ hybridisation probe |
| Identifier: | MGI:2450620 |
| Entity Detected: | Eomes, eomesodermin homolog (Xenopus laevis) ( MGI:1201683) |
| Notes: | The Eomes probe used in this study by Ciruna & Rossant, 1999 [PMID:10330500] is described as follows: "Novel T-box motifs were amplified by PCR from an E7.5 primitive streak cDNA library (generous gift from Dr. Jonathan Pearce) using degenerate primers encoding amino acid sequences GTEMIITK and NPFAKGFR. Amplification products were analyzed by PAGE and amplicons in the expected size range (about 500 bp) were eluted from gel slices, subcloned and sequenced. An isolated amplicon encoding the mEomes T-box was used as a probe for whole mount RNA in situ hybridizations. A mEomes 3' RACE product, containing sequence downstream of the T-box including 3' UTR, was also used as an in situ probe and gave identical results.
Editors Note: it is not specified which of these two probes was used in this actual sample. |
| Chemistry: | RNA |
| Strand: | antisense |
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