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EMAGE:3825

Nkx2-9 NK2 transcription factor related, locus 9 (Drosophila) ( MGI:1270158)
TS12 (8.5 dpc)
in situ hybridisation

Data Images
EMAGE:3825
Fig3E.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(98)00035-5] Mech Dev 73: 85-93, Pabst O; Herbrand H; Arnold HH, Nkx2-9 is a novel homeobox transcription factor which demarcates ventral domains in the developing mouse CNS. Copyright 1998

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
See EMAGE:3826 for sections F and G that are indicated in this image.
Expression Pattern Description
Spatial Annotation:
EMAGE:3825Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3825_wholemount_strong_3D_1.wlz
3825_wholemount_moderate_3D_1.wlz
3825_wholemount_weak_3D_1.wlz
3825_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3825_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
future brain
detected detected
regional
future spinal cord
detected detected
regional
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1270228
Entity Detected:Nkx2-9, NK2 transcription factor related, locus 9 (Drosophila) ( MGI:1270158)
Notes:The Nkx2-9 riboprobes used in this study by Pabst et al, 1998 [PMID:9545546] "were generated from an 1137-bp SmaI fragment containing nearly the entire Nkx2-9 cDNA, a 406-bp KpnI/SmaI fragment containing bases 731-1137 and a 409-bp PstI fragment containing bases 1-409". The MGI IDs for these fragments are: MGI:1270228, MGI:1270229 and MGI:1270230. Editors note: It is not specified which of these three probes was used in this actual ISH assay however the authors state that all gave similar results with some variations in signal intensities.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:8.5 dpc
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Pabst O; Herbrand H; Arnold HH, 1998 [PMID:9545546] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(98)00035-5] [ PMID:9545546] Pabst O, Herbrand H, Arnold HH 1998 Nkx2-9 is a novel homeobox transcription factor which demarcates ventral domains in the developing mouse CNS. Mech Dev (73):85-93
Links:MGI:3029967 same experiment
 EMAGE:3826 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI