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EMAGE:3826

Nkx2-9 NK2 transcription factor related, locus 9 (Drosophila) ( MGI:1270158)
TS13 (8.5 dpc)
in situ hybridisation

Data Images
EMAGE:3826 EMAGE:3826
Fig3F.Copyright: Reprinted with permission from Elsevier from [ [doi:10.1016/S0925-4773(98)00035-5] Mech Dev 73: 85-93, Pabst O; Herbrand H; Arnold HH, Nkx2-9 is a novel homeobox transcription factor which demarcates ventral domains in the developing mouse CNS. Copyright 1998 Fig3G.Copyright: Reprinted with permission from Elsevier from [ [doi:10.1016/S0925-4773(98)00035-5] Mech Dev 73: 85-93, Pabst O; Herbrand H; Arnold HH, Nkx2-9 is a novel homeobox transcription factor which demarcates ventral domains in the developing mouse CNS. Copyright 1998

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
See EMAGE:3825 for a wholemount view of the embryo that these sections were taken from. Image annotations: ba, branchial arches; ht, heart; nt, neural tube. Arrows: Nkx2-9 expression domains.
Expression Pattern Description
Spatial Annotation:
EMAGE:3826EMAGE:3826Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3826_voxel_strong_3D_1.wlz
3826_voxel_moderate_3D_1.wlz
3826_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3826_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
future brain
detected detected
regionalExpression is ventral and in small domains.
neural tube
detected detected
regionalExpression is ventral and in small domains.
neural tube floor plate
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1270228
Entity Detected:Nkx2-9, NK2 transcription factor related, locus 9 (Drosophila) ( MGI:1270158)
Notes:The Nkx2-9 riboprobes used in this study by Pabst et al, 1998 [PMID:9545546] "were generated from an 1137-bp SmaI fragment containing nearly the entire Nkx2-9 cDNA, a 406-bp KpnI/SmaI fragment containing bases 731-1137 and a 409-bp PstI fragment containing bases 1-409". The MGI IDs for these fragments are: MGI:1270228, MGI:1270229 and MGI:1270230. Editors note: It is not specified which of these three probes was used in this actual ISH assay however the authors state that all gave similar results with some variations in signal intensities.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:8.5 dpc
Theiler Stage:TS13
Mutations:none (wild-type)
Preparation:sectioned wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Pabst O; Herbrand H; Arnold HH, 1998 [PMID:9545546] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(98)00035-5] [ PMID:9545546] Pabst O, Herbrand H, Arnold HH 1998 Nkx2-9 is a novel homeobox transcription factor which demarcates ventral domains in the developing mouse CNS. Mech Dev (73):85-93
Links:MGI:3029967 same experiment
 EMAGE:3825 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI