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EMAGE:3827

Nkx2-9 NK2 transcription factor related, locus 9 (Drosophila) ( MGI:1270158)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:3827 EMAGE:3827 EMAGE:3827 EMAGE:3827
Fig3I.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(98)00035-5] Mech Dev 73: 85-93, Pabst O; Herbrand H; Arnold HH, Nkx2-9 is a novel homeobox transcription factor which demarcates ventral domains in the developing mouse CNS. Copyright 1998 Fig3H.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(98)00035-5] Mech Dev 73: 85-93, Pabst O; Herbrand H; Arnold HH, Nkx2-9 is a novel homeobox transcription factor which demarcates ventral domains in the developing mouse CNS. Copyright 1998 Fig3J.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(98)00035-5] Mech Dev 73: 85-93, Pabst O; Herbrand H; Arnold HH, Nkx2-9 is a novel homeobox transcription factor which demarcates ventral domains in the developing mouse CNS. Copyright 1998 Fig3K.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(98)00035-5] Mech Dev 73: 85-93, Pabst O; Herbrand H; Arnold HH, Nkx2-9 is a novel homeobox transcription factor which demarcates ventral domains in the developing mouse CNS. Copyright 1998

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
The wholemount stained embryo in H was subsequently sectioned. Section planes I, J and K are indicated in the wholemount view. Image annotations: fb, forebrain; hb, hindbrain; ht, heart; nt, neural tube. Arrows point to the expression domains. Editors Note: Based on morphology, it appears the authors have confused sections I and J and also mixed the labelling of the mb and hb in Fig 3J.
Expression Pattern Description
Spatial Annotation:
EMAGE:3827EMAGE:3827EMAGE:3827EMAGE:3827EMAGE:3827Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D mapping3D mappingspatial mappingwholemount mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3827_wholemount_strong_3D_4.wlz
3827_wholemount_moderate_3D_3.wlz
3827_wholemount_notDetected_3D_4.wlz
3827_voxel_moderate_3D_1.wlz
3827_voxel_notDetected_3D_1.wlz
3827_voxel_strong_3D_1.wlz
3827_voxel_moderate_3D_2.wlz
3827_voxel_notDetected_3D_2.wlz
3827_voxel_strong_3D_2.wlz
3827_voxel_notDetected_3D_3.wlz
3827_voxel_strong_3D_3.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3827_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future brain
detected detected
regionalExpression is in two parallel stripes along the brain, at the anterior and posterior end the stripes fuse to a 'V'-shape (Fig3I, 3J). Expression is in a more dorsal positition (Fig 3H).
neural tube floor plate
not detected not detected
homogeneous
neural tube
detected detected
regionalExpression has shifted from the floor plate to a more dorsal domain (Fig 3K).
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1270228
Entity Detected:Nkx2-9, NK2 transcription factor related, locus 9 (Drosophila) ( MGI:1270158)
Notes:The Nkx2-9 riboprobes used in this study by Pabst et al, 1998 [PMID:9545546] "were generated from an 1137-bp SmaI fragment containing nearly the entire Nkx2-9 cDNA, a 406-bp KpnI/SmaI fragment containing bases 731-1137 and a 409-bp PstI fragment containing bases 1-409". The MGI IDs for these fragments are: MGI:1270228, MGI:1270229 and MGI:1270230. Editors note: It is not specified which of these three probes was used in this actual ISH assay however the authors state that all gave similar results with some variations in signal intensities.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:sectioned wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Pabst O; Herbrand H; Arnold HH, 1998 [PMID:9545546] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(98)00035-5] [ PMID:9545546] Pabst O, Herbrand H, Arnold HH 1998 Nkx2-9 is a novel homeobox transcription factor which demarcates ventral domains in the developing mouse CNS. Mech Dev (73):85-93
Links:MGI:3029967 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI