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EMAGE:3849

Satb2 special AT-rich sequence binding protein 2 ( MGI:2679336)
TS16 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3849 EMAGE:3849 EMAGE:3849 EMAGE:3849 EMAGE:3849
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: three stars
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured red.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
branchial arch
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:SATB2_T7
Entity Detected:Satb2, special AT-rich sequence binding protein 2 ( MGI:2679336)
Sequence:sense strand is shown

>SATB2_T7
CAGCAGCTCCTCGTCCTTATACTCTGCCACGTCCACGGCGGAGCCCAGGTGCTCCTTCAGCTTGCCATGG
TGCTTCACGTGGTACCTCTGGTTCTGGAAGAACTTGATGATGGTGTGTTTGGGGAGATCCAGCTGGGCGG
AGAGTGTGTGGATGGCTTCCTGGTCGGGATAGAGGCCTACATCATGGATGAAGCTTTGAAGGATGCCCAG
GGCTTCCAAAGAGATCTTTGTGCGAGACCGAGGCTTTTTGGCACAGCTGTCTTCTGTTGGAGGAGGC
nt 56741445 - nt 56741721 of NC_000067.4
Notes:These primers were used to generate the in vitro transcription template by PCR of mouse whole genomic DNA. They contain T7 or T3 promotor sequences and amplify a region corresponding to a portion of the 3'UTR. Forward 5'-AATTAACCCTCACTAAAGGGCCTCCTCCAACAGAAGACA-3' (T3 sense) Reverse 5'-TAATACGACTCACTATAGGCAGCAGCTCCTCGTCCTTAT-3' (T7 antisense)
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD1
Age:10.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Jacqueline Ramsay, David R Fitzpatrick
Principal investigator:David R FitzPatrick, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Submitted by:Jacqueline Ramsay, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE