EMAGE mouse gene expression spatial database

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EMAGE:3864

Cited2 Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2 ( MGI:1306784)
TS12 (8 Somite no.)
in situ hybridisation

Data Images


Fig7F.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(98)00011-2] Mech Dev 72: 27-40, Dunwoodie SL; Rodriguez TA; Beddington RS, Msg1 and Mrg1, founding members of a gene family, show distinct patterns of gene expression during mouse embryogenesis. Copyright 1998	Fig7E.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(98)00011-2] Mech Dev 72: 27-40, Dunwoodie SL; Rodriguez TA; Beddington RS, Msg1 and Mrg1, founding members of a gene family, show distinct patterns of gene expression during mouse embryogenesis. Copyright 1998	Fig7K.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(98)00011-2] Mech Dev 72: 27-40, Dunwoodie SL; Rodriguez TA; Beddington RS, Msg1 and Mrg1, founding members of a gene family, show distinct patterns of gene expression during mouse embryogenesis. Copyright 1998

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

(F) Lateral view of embryo in (E), presumptive septum transversum; black arrowhead. Cranial neuroectoderm in presumptive midbrain and hindbrain (black arrows). (E) The foregut diverticulum is indicated with an unfilled arrowhead; 1 and 2 = two bands of expression adjacent to the invaginating foregut endoderm. Site 1 is rostral (black arrowhead) and corresponds to the future septum transversum. Site 2 is caudal (white arrow). (K) Midline sagittal section of embryo shown in (E). The myocardium has almost completely engulfed the endocardium bringing the Mrg1 expressing sites 1 (black arrowhead) and 2 (white arrow) into close proximity. Cited2 (Mrg1) is still strongly expressed, at site 1, in the septum transversum (st) and adjacent myocardium (m) but only low levels of transcript persist at site 2. g = foregut.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

3864_wholemount_strong_3D_1.wlz

3864_wholemount_possible_3D_1.wlz

3864_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3864_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trunk mesenchyme

detected

regional

Expression in two bands corresponding to the site where the future myocardium adjoins the splanchnic mesoderm, the site of the future septum transversum and at a more caudal site.

cardiovascular system

detected

regional

Expression in the presumptive septum transversum.

future midbrain

detected

future hindbrain

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:324064
Entity Detected:	Cited2, Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2 ( MGI:1306784)
Sequence:	sense strand is shown >MGI:324064 CAGCGCGGGCGGCACGGCNGGTGGCAGCACCATGCCCGCCTCGGTGGCTCACGTCCCCGCGGCAATGCTG CCGCCCAATGTCATAGACACTGATTTCATCGACGAGGAAGTGCTTATGTCCTTAGTGATAGAAATGGGTT TGGACCGCATCAAGGAGCTGCCCGAACTCTGGCTGGGCAAAATGAGTTTGATTTTATGACGGACTTCGTG TGCAAGCAGCAGCCCAGCAGAGTCAGCTGTTGACTCGGTTAACCTCGCAGGCGGAAAGAAATCACCCTCC CCACCCTAGCCCCACCCCCAACTTCTTCGGTGTGAATTA
	nt 1 - nt 320 of AA117983.1
Notes:	The Cited2 (Mrg1) probe used in this study by Dunwoodie et al, 1998 [PMID:9533950] was transcribed from either clone AA117983 (IMAGE:537128) or W15571 (IMAGE:333087). The authors state that both riboprobes revealed the same pattern of Mrg1 transcript accumulation.
Chemistry:	RNA
Strand:	antisense

Specimen

Organism:	mouse
Strain:	involves: C57BL/6 * DBA
Age:	8 Somite no.
Theiler Stage:	TS12
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:

4% paraformaldehyde

General Information

Authors:	Dunwoodie SL; Rodriguez TA; Beddington RS, 1998 [PMID:9533950] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/S0925-4773(98)00011-2] [ PMID:9533950] Dunwoodie SL, Rodriguez TA, Beddington RS 1998 Msg1 and Mrg1, founding members of a gene family, show distinct patterns of gene expression during mouse embryogenesis. Mech Dev (72):27-40
Links:	MGI:3046868 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source