EMAGE mouse gene expression spatial database

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EMAGE:3868

Dkk1 dickkopf homolog 1 (Xenopus laevis) ( MGI:1329040)
TS14 (9.0 dpc)
in situ hybridisation

Data Images


Fig1A.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(99)00194-X] Mech Dev 89: 151-3, Grotewold L; Theil T; Ruther U, Expression pattern of Dkk-1 during mouse limb development. Copyright 1999

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Arrow indicates the ventral diencephalon.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

3868_wholemount_strong_3D_1.wlz

3868_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3868_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

unsegmented mesenchyme

detected

future diencephalon

detected

regional

Expression in ventral diencephalon.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:1329444
Entity Detected:	Dkk1, dickkopf homolog 1 (Xenopus laevis) ( MGI:1329040)
Sequence:	sense strand is shown >MGI:1329444 GACCCACGCGTCCGTGCCTGTTTGCGTCCTTCGGAGATGATGGTTGTGTGTGCACCGGCAGCTGTCCGGT TCTTGGCCGTGTTTACAATGATGGCTCTCTGCAGCCTCCCTCTGCTAGGAGCCAGTGCCACCTTGAACTC AGTTCTCATCAATTCCAACGCGATCAAGAACCTGCCCCCACCGCTGGGTGGTGCTGGGGGGCAGCCGGGC TCTGCTGTCAGTGTGGCGCCGGGAGTTCTCTATGAGGGCGGGAACAAGTACCAGACTCTTGACAACTACC AGCCCTACCCTTGCGCTGAAGATGAGGAGTGCGGCTCTGACGAGTACTGCTCCAGCCCCAGCCGCGGGGC AGCCGGCGTCGGAGGTGTACAGATCTGTCTGGCTTGCCGAAAGCGCAGGAAGCGCTGCATGACGCACGCT ATGTGCTGCCCCGGGAACTACTGCAAAAATGGAATATGCATGCCCTCTGACCACAGCCATTTTCCTCGAG GGGAAATTGAGGAAAGCATCATTGAAAACCTTGGTAATGACCACAACGCCGCCGCGGGGGATGGATATCC CAGAAGAACCACACTGACTTCAAAAATATATCACACCAAAGGACAAGAAGGCTCCGTCTGCCTCCGATCA TCAGACTGTGCCGCAGGGCTGTGTTGTGCAAGACACTTCTGGTCCAAGATCTGTAAACCTGTCCTTAAAG AAGGTCAGGTGTGCACCAAGCACAAACGGAAAGGCTCCCACGGGCTGGAGATATTCCAGCGCTGTTACTG CGGGGAAGGCCTGGCTTGCAGGATACAGAAAGATCACCATCAAGCCAGCAATTCTTCTAGGCTCCACACC TGCCAGAGACACTAAACCGACAGTCTAAATATGATGGACTCTTTTTATCTAATATATGCTACGAAAATCC TTTATGATTTGTCAGCTCAATCCCAAGGATGTAGGAATCTTCAGTGTGTAATTAAGCATTCCGACAATAC TTTCCAAAAGCTCTGGAGTGTAAGGACTTTGTTTCTTGATGGAACTCCCCTGTGATTGCAGTAAATTACT GTGTTGTAAATCCTCAGTGTGGCACTTACCTGTAAATGCAGCAAAACTTTTAATTATTTTTCTAGAGGTG TGGTACATTGCCTTGTTTCTCTTGCATGTAAATTTTTTTTGTACACGGTTGATTGTCTTGACTCATAAAT ATTCTATATTGGAGTAGAAAAAAAAAAAAAAAAAAA
	nt 1 - nt 1226 of AF030433.1
Notes:	The Dkk1 probe used in this study by Grotewold et al, 1999 [PMID:10559490] is described as being made from "Dkk-1 (Glinka et al., 1998 [PMID:9450748] )". Glinka et al generated an in situ hybridisation probe from a clone that was isolated from a mouse embryonic day 9 cDNA library by the RZPD (clone MPMGc559P2481Q3). AF030433.1 is the accompanying GenBank entry for the insert sequence of this clone.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	involves: C3H * C57BL/6
Age:	9.0 dpc
Theiler Stage:	TS14
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:	4% paraformaldehyde
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Grotewold L; Theil T; Ruther U., 1999. [PMID:10559490] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/S0925-4773(99)00194-X] [ PMID:10559490] Grotewold L, Theil T, Ruether U 1999 Expression pattern of Dkk-1 during mouse limb development. Mech Dev (89):151-3
	[ doi:10.1038/34848] [ PMID:9450748] Glinka A, Wu W, Delius H, Monaghan AP, Blumenstock C, Niehrs C 1998 Dickkopf-1 is a member of a new family of secreted proteins and functions in head induction. Nature (391):357-62
Links:	MGI:2388194 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source