EMAGE mouse gene expression spatial database

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EMAGE:3875

Dlx2 distal-less homeobox 2 ( MGI:94902)
TS17 (10.5 dpc)
in situ hybridisation

Data Images


Raw signal output, as movie.	All sections along the X-axis, as movie.	All sections along the Y-axis, as movie.	All sections along the Z-axis, as movie.	Photograph prior to 3D imaging.

Photograph prior to 3D imaging.	Sample morphology following 3D imaging, as movie

View 3D opt image:
Download 3D images in woolz format
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured red. NOTE: This embryo has considerable reagent trapping within the neural tube.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

3875_wholemount_strong_3D_1.wlz

3875_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3875_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	DLX2_T7
Entity Detected:	Dlx2, distal-less homeobox 2 ( MGI:94902)
Sequence:	sense strand is shown >DLX2_T7 TACGTCGCAGCTTTCACAACTCGGGGGCGGTTTACAAAAAAGTCTGAAAAGATGAGAAATTTTTATACAA ATAAATGTGGGGAAATCTGCACAGACACCCTTATTTACAAACTCTGCATCGAAGTTCGAGCTAATAGTCC TTAATTCATCTTAAAAAAGATAATTTAAACACATTTTTTGCAGCGTCCACATATTAAAAAAATCAGTTTT TTTTTAACATCAAAATGAGGTCATCCGCAAAGGCACCTAAACTTTTAAAAAAATAAAAATAAAAACCCTC CACTGGCAATGGATGAAGGAGCTACGGGAGTGTTCCTGGGGAAAGTGGGAGGCGGGAAGAGTTGGGAGAG CTGGGAATTCTCCCCCATGAGCCCCCAGGAGGGGAAAAAACCGGCGGGATCCCGGGGGTCACGGGGGTAG GTGATAGGGTGGAGTAGGACCCAGGAGGTCAATAACCGAAGCGGACTGGAGCTGAGGCCTGGAGCAGGAG AGAAAAAAGGAGCCTCTGAGAGCC
	nt 71344461 - nt 71344974 of NC_000068.5
Notes:	These primers were used to generate the in vitro transcription template by PCR of mouse whole genomic DNA. They contain T7 or T3 promotor sequences and amplify a region corresponding to a portion of the 3'UTR. T3 forward primer (sense) 5'-AATTAACCCTCACTAAAGGGGCTCTCAGAGGCTCCTTTT-3' T7 reverse primer (antisense) 5'-TAATACGACTCACTATAGGTACGTCGCAGCTTTCACAAC-3'
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD1
Age:	10.5 dpc
Theiler Stage:	TS17
Mutations:	none (wild-type)
Preparation:	opt

Procedures

Fixation:	4% paraformaldehyde
Embedding:	agarose
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Jacqueline Ramsay, David R Fitzpatrick
Principal investigator:	David R FitzPatrick, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Submitted by:	Jacqueline Ramsay, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Experiment type:	screen
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source