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EMAGE:3885

Pdgfa platelet derived growth factor, alpha ( MGI:97527)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3885
Figure 1G. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2005.04.009] Gene Expr Patterns 6: 94-101, Machka C; Kersten M; Zobawa M; Harder A; Horsch M; Halder T; Lottspeich F; Hrabe de Angelis M; Beckers J, Identification of Dll1 (Delta1) target genes during mouse embryogenesis using differential expression profiling. Copyright 2005.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: Arrows indicate segmented expression in dermomyotomes.
Expression Pattern Description
Spatial Annotation:
EMAGE:3885Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3885_wholemount_strong_3D_1.wlz
3885_wholemount_moderate_3D_1.wlz
3885_wholemount_weak_3D_1.wlz
3885_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3885_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
regionalExpression in the dermomyotome.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1336703
Entity Detected:Pdgfa, platelet derived growth factor, alpha ( MGI:97527)
Notes:The Pdgfa probe used in this study by Machka et al., 2005 [PMID:15979417] is indicated as that originally described by Bostrom et al., 1996 [PMID:8681381] i.e. as "a 900 bp EcoRI fragment cloned in pGEM-1".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Machka C; Kersten M; Zobawa M; Harder A; Horsch M; Halder T; Lottspeich F; Hrabe de Angelis M; Beckers J, 2005 [PMID:15979417] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.modgep.2005.04.009] [ PMID:15979417] Machka C, Kersten M, Zobawa M, Harder A, Horsch M, Halder T, Lottspeich F, Hrabe de Angelis M, Beckers J 2005 Identification of Dll1 (Delta1) target genes during mouse embryogenesis using differential expression profiling. Gene Expr Patterns (6):94-101
 [ doi:10.1016/S0092-8674(00)81270-2] [ PMID:8681381] Bostrom H, Willetts K, Pekny M, Leveen P, Lindahl P, Hedstrand H, Pekna M, Hellstrom M, Gebre-Medhin S, Schalling M, Nilsson M, Kurland S, Tornell J, Heath JK, Betsholtz C 1996 PDGF-A signaling is a critical event in lung alveolar myofibroblast development and alveogenesis. Cell (85):863-73
Links:MGI:3617192 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI