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EMAGE:3930

Hoxa2 homeobox A2 ( MGI:96174)
TS15 (8.75-9.25 dpc)
in situ hybridisation

Data Images
EMAGE:3930
Fig1D(wt). Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1999.9527] Dev Biol 217: 10-24, Clouthier DE; Williams SC; Yanagisawa H; Wieduwilt M; Richardson JA; Yanagisawa M, Signaling pathways crucial for craniofacial development revealed by endothelin-A receptor-deficient mice. Copyright 2000

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: Pharyngeal arches are outlined in yellow; Yellow arrows denote migrating neural crest cells; h - heart; op - otic placode; 1 - 1st branchial arch; 2 - 2nd branchial arch, bracket denotes rhombomere (r)2-r4.
Expression Pattern Description
Spatial Annotation:
EMAGE:3930Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3930_wholemount_strong_3D_1.wlz
3930_wholemount_possible_3D_1.wlz
3930_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3930_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
2nd branchial arch mesenchyme derived from neural crest
detected detected
regionalExpression is in neural crest cells streaming into branchial arch 2 from r4.
rhombomere 02
detected detected
rhombomere 03
detected detected
rhombomere 04
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1346652
Entity Detected:Hoxa2, homeobox A2 ( MGI:96174)
Sequence:sense strand is shown

>MGI:1346652
CCTTCTGGAGAGGGAAGGGTACACTTTTCAGCAAAATGCGCTCTCTCAACAGCAGGCTCCCAATGGACAC
AATGGCGACTCCCAAACTTTCCCAGTTTCGCCTTTAACCAGCAATGAGAAAAATTTGAAACATTTTCAGC
ACCAGTCACCCACTGTTCCTAACTGCTTGTCAACAATGGGCCAGAACTGTGGAGCTGGCCTAAACAATGA
CAGTCCCGAGGCCATCGAGGTCCCCTCTTTGCAGGACTTCAATGTTTTCTCCACAGATTCCTGCCTGCAG
CTTTCAGATGCACTGTCGCCCAGCTTGCCTGGCTCCCTGGACAGTCCTGTAGAT
nt 901 - nt 1234 of NM_010451.1
Notes:The Hoxa2 probe used in this study by Clouthier et al., 2000 [PMID:10625532] is indicated as that previously described by Manley and Capecchi, 1995 [PMID:7635047] i.e. "a 338 base pair ApaI-EcoRV fragment from the coding region 3' of the homeobox." Editors note: The probe start-end coordinates w.r.t. the Hoxa2 RefSeq cDNA sequence NM_010451.1 were deduced from this information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:8.75-9.25 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Clouthier DE; Williams SC; Yanagisawa H; Wieduwilt M; Richardson JA; Yanagisawa M, 2000 [PMID:10625532] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.1999.9527] [ PMID:10625532] Clouthier DE, Williams SC, Yanagisawa H, Wieduwilt M, Richardson JA, Yanagisawa M 2000 Signaling pathways crucial for craniofacial development revealed by endothelin-A receptor-deficient mice. Dev Biol (217):10-24
 [ PMID:7635047] Manley NR, Capecchi MR 1995 The role of Hoxa-3 in mouse thymus and thyroid development. Development (121):1989-2003
Links:MGI:2135641 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI