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EMAGE:3932

Hoxa3 homeobox A3 ( MGI:96175)
TS14 (8.75 dpc)
in situ hybridisation

Data Images
EMAGE:3932
Fig8B. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2001.0447] Dev Biol 240: 15-31, Watari N; Kameda Y; Takeichi M; Chisaka O, Hoxa3 regulates integration of glossopharyngeal nerve precursor cells. Copyright 2001

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: dotted line - otic vesicle; arrowheads - expression of Hoxa3 in neural crest cells located from the most dorsal region towards the (IXth nerve) placode; dark bracket indicates rhombomere 6; light bracket indicates branchial arch 3.
Expression Pattern Description
Spatial Annotation:
EMAGE:3932Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3932_wholemount_strong_3D_1.wlz
3932_wholemount_moderate_3D_1.wlz
3932_wholemount_weak_3D_1.wlz
3932_wholemount_possible_3D_1.wlz
3932_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3932_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
3rd branchial arch mesenchyme derived from neural crest
strong strong
regionalExpression is in migrating neural crest cells.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2155925
Entity Detected:Hoxa3, homeobox A3 ( MGI:96175)
Notes:The Hoxa3 probe used in this study by Watari et al., 2001 [PMID:11784044] is described as follows: "Hoxa3 antisense probe was transcribed from a HindIII-AatI fragment of Hoxa3 cDNA, which is 5' of the homeodomain-coding sequence." Editors note: Restriction site searching of the Hoxa3 RefSeq cDNA NM_010452.2 and other Hoxa3 sequences (e.g. Y11717.1) for HindIII and AatI sites finds only one potential HindIII/AatI fragment, which is only 45nt in length. It seems unlikely that a probe is this length was used - further information required to clarify.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:8.75 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Watari N; Kameda Y; Takeichi M; Chisaka O, 2001 [PMID:11784044] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2001.0447] [ PMID:11784044] Watari N, Kameda Y, Takeichi M, Chisaka O 2001 Hoxa3 regulates integration of glossopharyngeal nerve precursor cells. Dev Biol (240):15-31
Links:MGI:2156500 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI