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EMAGE:395

Wnt10b wingless related MMTV integration site 10b ( MGI:108061)
TS22 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:395 EMAGE:395
Figure 3B of Cygan et al., 1997 [PMID:9362463] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 3D of Cygan et al., 1997 [PMID:9362463] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Notes:
Image B is a dorsal view of the forelimb; Image D is a ventral view of the forelimb.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
forelimb digit 1 skin
detected detected
regionalDiffuse expression in the epithelium of the digits.
forelimb digit 3 skin
detected detected
regionalDiffuse expression in the epithelium of the digits.
forelimb digit 2 skin
detected detected
regionalDiffuse expression in the epithelium of the digits.
forelimb digit 4 skin
detected detected
regionalDiffuse expression in the epithelium of the digits.
forelimb digit 5 skin
detected detected
regionalDiffuse expression in the epithelium of the digits.
forearm skin
detected detected
regionalExpression on the dorsal surface of the autopod in the developing hair follicles.
hand rest of skin
detected detected
regionalExpression on the dorsal surface of the autopod in the developing hair follicles. (Image D) Expression on the ventral surface in the epithelium and mesenchyme of the devloping footpads.
hand
detected detected
regional(Image D) Expression on the ventral surface in the epithelium and mesenchyme of the devloping footpads.
hand mesenchyme
detected detected
regional(Image D) Expression on the ventral surface in the epithelium and mesenchyme of the devloping footpads.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1196544
Entity Detected:Wnt10b, wingless related MMTV integration site 10b ( MGI:108061)
Notes:The Wnt10b probe used in this study by Cygan et al., 1997 [PMID:9362463] was described as follows: "A probe for Wnt-10b was synthesized from a 400 bp PCR fragment cloned as described by Gavin et al., 1990 [PMID:2279700] , linearized with EcoRI and transcribed with T3 polymerase."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:14.5 dpc
Theiler Stage:TS22
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Cygan et al., 1997 [PMID:9362463] Indexed by GXD.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9362463] Cygan JA, Johnson RL, McMahon AP 1997 Novel regulatory interactions revealed by studies of murine limb pattern in Wnt-7a and En-1 mutants. Development (124):5021-32
 [ doi:10.1101/gad.4.12b.2319] [ PMID:2279700] Gavin BJ, McMahon JA, McMahon AP 1990 Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development. Genes Dev (4):2319-32
Links:MGI:1933279 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI