Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:3968

Myod1 myogenic differentiation 1 ( MGI:97275)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3968
Figure 3A of Wilson-Rawls et al., 1999 [PMID:10556048] . Copyright: This image is from Wilson-Rawls et al. Development 126:5217-5229 (1999), and is displayed with the permission of The Company of Biologist Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: the medial and lateral boundaries of MyoD transcription in the myotome is denoted by the red and black arrowheads, respectively. The green arrowhead points to MyoD-positive cells in the branchial arches.
Expression Pattern Description
Spatial Annotation:
EMAGE:3968Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3968_wholemount_strong_3D_1.wlz
3968_wholemount_possible_3D_1.wlz
3968_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3968_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
branchial arch
detected detected
trunk somite
detected detected
regionalExpression in dermomyotomes.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1309772
Entity Detected:Myod1, myogenic differentiation 1 ( MGI:97275)
Sequence:sense strand is shown

>MGI:1309772
GCGTCCAGCCCGCGCTCCAACTGCTCTGATGGCATGATGGATTACAGCGGCCCCCCAAGCGGCCCCCGGC
GGCAGAATGGCTACGACACCGCCTACTACAGTGAGGCGGTGCGCGAGTCCAGGCCAGGGAAGAGTGCGGC
TGTGTCGAGCCTCGACTGCCTGTCCAGCATAGTGGAGCGCATCTCCACAGACAGCCCCGCTGCGCCTGCG
CTGCTTTTGGCAGATGCACCACCAGAGTCGCCTCCGGGTCCGCCAGAGGGGGCATCCCTAAGCGACACAG
AACAGGGAACCCAGACCCCGTCTCCCGACGCCGCCCCTCAGTGTCCTGCAGGCTCAAACCCCAATGCGAT
TTATCAGGTGCTTTGAGAGATCGACTGCAGCAGCAGAGGGCGCACCACCGTAGGCACTCCTGGGGATGGT
GCCCCTGGTTCTTCACGCCCAAAAGATGAAGCTTAAATGACACTCTTCCCAACTGTCCTTTCGAAGCCGT
TCTTCCAGAGGGAAGGGAAGAGCAGAAGTCTGTCCTAGATCCAGCCCCAAAGAAAGGACATAGTCCTTTT
TGTTGTTGTTGTTGTAGTCCTTCAGTTGTTTGTTTGTTTTTTCATGCGGCTCACAGCGAAGGCCACTTGC
ACTCTGGCTGCACCTCACTGGGCCAGAGCTGATCCTTGAGTGGCCAGGCGCTCTTCCTTTCCTCATAGCA
CAGGGGTGAGCCTTGCACACCTAAGCCCTGCCCTCCACATCCTTTTGTTTGTCACTTTCTGGAGCCCTCC
TGGCACCCACTTTTCCCCACAGCTTGCGGAGGCCACTCAGGTCTCAGGTGTAACAGGTGTAACCATACCC
CACTCTCCCCCTTCCCGCGGTTCAGGACCACTTATTTTTTTATATAAGACTTTTGTAATCTATTCGTGTA
AATAAGAGTTGCTTGGCCAGAGCGGGAGCCCCTTGGGCTATATTTATCTCCCAGGCATGCTGTGTAGTGC
AACAAAAACTTTGTATGTTTTATTCCTCAAGCGGGCGAGTCAGGTGTTGGAAATAA
nt 783 - nt 1818 of NM_010866.1
Notes:The Myod1 (MyoD) probe used in this study by Wilson-Rawls et al., 1999 [PMID:10556048] is indicated as that originally described by Sassoon et al., 1989 [PMID:2552320] as nt 751-1785 of the sequence shown in Fig8 of Davis et al., [PMID:3690668] .
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:involves: 129S4/SvJae
Age:10.5 dpc
Theiler Stage:TS17
Mutations:Myf5+/Myf5tm1Thbr
Preparation:wholemount
Procedures
Fixation:10% Formalin
General Information
Authors:Wilson-Rawls J; Hurt CR; Parsons SM; Rawls A, 1999 [PMID:10556048] .
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10556048] Wilson-Rawls J, Hurt CR, Parsons SM, Rawls A 1999 Differential regulation of epaxial and hypaxial muscle development by paraxis. Development (126):5217-29
 [ doi:10.1038/341303a0] [ PMID:2552320] Sassoon D, Lyons G, Wright WE, Lin V, Lassar A, Weintraub H, Buckingham M 1989 Expression of two myogenic regulatory factors myogenin and MyoD1 during mouse embryogenesis. Nature (341):303-7
 [ doi:10.1016/0092-8674(87)90585-X] [ PMID:3690668] Davis RL, Weintraub H, Lassar AB 1987 Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell (51):987-1000
Links:MGI:1930192 same experiment
 MGI:1857219 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI