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EMAGE:397

Wnt1 wingless-related MMTV integration site 1 ( MGI:98953)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:397
Figure 3E of Wassarman et al., 1997 [PMID:9247335] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Notes:
Image annotations: mb - midbrain; isc - isthmic constriction.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
hindbrain
detected detected
regionalExpression in a transverse band at the posterior end of the midbrain. Expressed in a stripe along the dorsal midline of the midbrain and of the hindbrain posterior to the cerebellar anlage.
midbrain
detected detected
regionalExpression in a transverse band at the posterior end of the midbrain. Expressed in a stripe along the dorsal midline of the midbrain and of the hindbrain posterior to the cerebellar anlage.
rhombomere 01
not detected not detected
rhombomere 02
not detected not detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1331398
Entity Detected:Wnt1, wingless-related MMTV integration site 1 ( MGI:98953)
Notes:The Wnt1 probe used in this study by Wassarman et al., 1997 [PMID:9247335] is described as being that previously published by McMahon et al., 1992 [PMID:1534034] . McMahon et al describe three Wnt1 in situ probes.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BM purple
General Information
Authors:Wassarman et al., 1997 [PMID:9247335] Indexed by GXD
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9247335] Wassarman KM, Lewandoski M, Campbell K, Joyner AL, Rubenstein JL, Martinez S, Martin GR 1997 Specification of the anterior hindbrain and establishment of a normal mid/hindbrain organizer is dependent on Gbx2 gene function. Development (124):2923-34
 [ doi:10.1016/0092-8674(92)90222-X] [ PMID:1534034] McMahon AP, Joyner AL, Bradley A, McMahon JA 1992 The midbrain-hindbrain phenotype of Wnt-1-/Wnt-1- mice results from stepwise deletion of engrailed-expressing cells by 9.5 days postcoitum. Cell (69):581-95
Links:MGI:1331429 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI