EMAGE mouse gene expression spatial database

EMAGE:3971

Etv4 ets variant gene 4 (E1A enhancer binding protein, E1AF) ( MGI:99423)
TS15 (9.5 dpc)
in situ hybridisation

Data Images


Figure 3E of Rallis et al., 2003 [PMID:12736217] . Copyright: This image is from Rallis et al., Development 130:2741-2751 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Black arrow indicates forelimb bud. Red arrow indicates intermediate mesoderm.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb bud

detected

trunk mesenchyme

detected

regional

Authors report expression in the intermediate mesenchyme.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:2670122
Entity Detected:	Etv4, ets variant gene 4 (E1A enhancer binding protein, E1AF) ( MGI:99423)
Sequence:	sense strand is shown >MGI:2670122 ACACCTTCTGCAGCAAATCTCCCGGAAATGGGAGCTTGGGCGAAGCGCTGATGGTCCCGCAGGGAAAGCT CATGGACCCGGGCTCCCTGCCGCCTTCCGACTCAGAAGATCTCTTCCAGGACCTCAGTCACTTCCAAGAG ACGTGGCTCGCAGAAGCTCAGGTACCGGACAGTGATGAGCAGTTTGTTCCTGATTTCCATTCAGAAAACT TAGCTTTCCATAGCCCCACCACCAGGATCAAGAAGGAACCCCAGAGTCCCCGCACAGACCCCGCCCTGTC CTGCAGCAGGAAGCCACCACTCCCCTACCACCATGGAGAGCAGTGCCTTTACTCCAGACAAATCGCCATC AAGTCCCCCGCTCCCGGTGCCCCTGGACAGTCGCCCCTGCAGCCCTTTTCCAGGGCAGAACAGCAGCAGA GCCTCCTGAGAGCCTCCAGCTCTTCCCAGTCCCACCCTGGCCACGGGTACCTTGGTGAGCACAGCTCCGT CTTCCAGCAGCCCGTGGACATGTGCCACTCCTTCACATCTCCTCAGGGAGGGGGCCGGGAACCTCTCCCA GCCCCCTATCAACACCAACTGTCGGAGCCCTGC
	nt 397 - nt 989 of X63190.1
Notes:	The Etv4 (Pea3) probe used in this study by Rallis et al., 2003 [PMID:12736217] was originally described by Chotteau-Lelievre et al., 1997 [PMID:9285689] and was generated by PCR using the primers 5'-CACCCTTCTGCAGCAAATCTCCCGG-3' and 5'-GCAGGGCTCCGACAGTTGGTGTTG-3', producing "593 bp (nt 397 to nt 989; (Xin et al., 1992 [PMID:1547944] )". Editors note: X63190.1 is the accompanying GenBank submission by Xin et al for their paper.
Chemistry:	RNA
Strand:	antisense

Specimen

Procedures

Fixation:

4% paraformaldehyde

General Information

Authors:	Rallis C; Bruneau BG; Del Buono J; Seidman CE; Seidman JG; Nissim S; Tabin CJ; Logan MP, 2003 [PMID:12736217] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1242/dev.00473] [ PMID:12736217] Rallis C, Bruneau BG, Del Buono J, Seidman CE, Seidman JG, Nissim S, Tabin CJ, Logan MP 2003 Tbx5 is required for forelimb bud formation and continued outgrowth. Development (130):2741-51
	[ doi:10.1038/sj.onc.1201261] [ PMID:9285689] Chotteau-Lievre A, Desbiens X, Pelczar H, Defossez PA, de Launoit Y 1997 Differential expression patterns of the PEA3 group transcription factors through murine embryonic development. Oncogene (15):937-52
	[ doi:10.1101/gad.6.3.481] [ PMID:1547944] Xin JH, Cowie A, Lachance P, Hassell JA 1992 Molecular cloning and characterization of PEA3, a new member of the Ets oncogene family that is differentially expressed in mouse embryonic cells. Genes Dev (6):481-96
Links:	MGI:2677746 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source