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EMAGE:3973

Gsc goosecoid homeobox ( MGI:95841)
TS17 (10.5 dpc)
immunohistochemistry

Data Images
EMAGE:3973
Figure 4A of Belo et al., 1998 [PMID:9533949] . Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(97)00204-9] Mech Dev 1998 Mar;72(1-2):15-25, Belo JA; Leyns L; Yamada G; De Robertis EM, The prechordal midline of the chondrocranium is defective in Goosecoid-1 mouse mutants. Copyright 1998.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:3973Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3973_wholemount_strong_3D_1.wlz
3973_wholemount_moderate_3D_1.wlz
3973_wholemount_possible_3D_1.wlz
3973_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3973_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
1st branchial arch
detected detected
2nd branchial arch
detected detected
forelimb bud
detected detected
hindlimb bud
detected detected
body-wall mesenchyme
detected detected
fronto-nasal process
detected detected
regionalExpression was detected in the neural crest of the frontonasal mass.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:3699916
Entity Detected:Gsc, goosecoid homeobox ( MGI:95841)
Notes:The anti-Gsc antisera used in this study by Belo et al., 1998 [PMID:9533949] was made as such: "Gsc-1 specific antigens were generated by subcloning exon 1 in the pGEX-2T expression vector, creating a fusion protein with glutathione-S-transferase (Smith and Johnson, 1988 [PMID:3047011] ). GST-Gsc-1 fusion protein was purified and polyclonal antisera raised according to Gamer and Wright, 1995 [PMID:7556900] . Wholemount immunostaining was as described by Gamer and Wright, 1993 [PMID:7902125] . Embryos were fixed in Dent's fixative. The resulting antibodies were of low titer and had to be used at a dilution of 1:2-1:5".
Antibody Type:polyclonal
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:Dent's fixative
General Information
Authors:Belo JA; Leyns L; Yamada G; De Robertis EM, 1998 [PMID:9533949] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(97)00204-9] [ PMID:9533949] Belo JA, Leyns L, Yamada G, De Robertis EM 1998 The prechordal midline of the chondrocranium is defective in Goosecoid-1 mouse mutants. Mech Dev (72):15-25
 [ doi:10.1016/0378-1119(88)90005-4] [ PMID:3047011] Smith DB, Johnson KS 1988 Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene (67):31-40
 [ doi:10.1006/dbio.1995.1275] [ PMID:7556900] Gamer LW, Wright CV 1995 Autonomous endodermal determination in Xenopus: regulation of expression of the pancreatic gene XlHbox 8. Dev Biol (171):240-51
Links:MGI:3699924 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI