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EMAGE:3974

Cxcl12 chemokine (C-X-C motif) ligand 12 ( MGI:103556)
TS20 (12.5 dpc)
in situ hybridisation

Data Images
EMAGE:3974
Figure 1A. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2004.10.007] Gene Expr Patterns 5: 317-22, Daniel D; Rossel M; Seki T; Konig N, Stromal cell-derived factor-1 (SDF-1) expression in embryonic mouse cerebral cortex starts in the intermediate zone close to the pallial-subpallial boundary and extends progressively towards the cortical hem. Copyright 2005.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Note: The plane of section (100 mm thick Vibratome sections) is perpendicular to the rostrocaudal axis of the forebrain. The arrow indicates the pallial-subpallial boundary. Dorsal is at top, lateral at left. EGE, external ganglionic eminence; MGE, median ganglionic eminence. Scale bar: 400 microns.
Expression Pattern Description
Spatial Annotation:
EMAGE:3974EMAGE:3974Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3974_voxel_strong_3D_1.wlz
3974_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3974_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
telencephalon
detected detected
regionalExpression is in a small part of the intermediate zone close to the pallial-subpallial boundary, absent in the subpallium.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3576595
Entity Detected:Cxcl12, chemokine (C-X-C motif) ligand 12 ( MGI:103556)
Notes:The Cxcl12 (SDF-1 alpha) probe template used in this study by Daniel et al., 2005 [PMID:15661637] is described as being provided by Dr Kathleen McGrath (University of Rochester, NY).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:OF1
Age:12.5 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Daniel D; Rossel M; Seki T; Konig N, 2005 [PMID:15661637] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.modgep.2004.10.007] [ PMID:15661637] Daniel D, Rossel M, Seki T, Konig N 2005 Stromal cell-derived factor-1 (SDF-1) expression in embryonic mouse cerebral cortex starts in the intermediate zone close to the pallial-subpallial boundary and extends progressively towards the cortical hem. Gene Expr Patterns (5):317-22
Links:MGI:3576598 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI