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EMAGE:3979

Zic3 zinc finger protein of the cerebellum 3 ( MGI:106676)
TS10 (mid-streak Downs & Davies)
in situ hybridisation

Data Images
EMAGE:3979
Figure 2G. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2004.03.003] Gene Expr Patterns 4: 505-11, Elms P; Scurry A; Davies J; Willoughby C; Hacker T; Bogani D; Arkell R, Overlapping and distinct expression domains of Zic2 and Zic3 during mouse gastrulation. Copyright 2004.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: P indicates level of section in EMAGE:3980. Anterior to left. Arrow points to expression in the ectoderm of the posterior amniotic fold.
Expression Pattern Description
Spatial Annotation:
EMAGE:3979Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3979_wholemount_strong_3D_1.wlz
3979_wholemount_moderate_3D_1.wlz
3979_wholemount_weak_3D_1.wlz
3979_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3979_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo ectoderm
weak weak
regionalExpression is in the anterior half.
embryo mesoderm
detected detected
regionalExpression is encircling the embryonic portion of the egg cylinder.
amniotic fold ectoderm
detected detected
regionalExpression is in ectoderm of the posterior amniotic fold.
extraembryonic component
not detected not detected
homogeneousThe mesoderm that moves into the extra embryonic region does not contain Zic3 transcripts
definitive endoderm
detected detected
regionalExpression is in anterior definitive endoderm: see accompanying section (Fig2P) in EMAGE:3980.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3526556
Entity Detected:Zic3, zinc finger protein of the cerebellum 3 ( MGI:106676)
Sequence:sense strand is shown

>MGI:3526556
ATTGAATCCCTTCGGGGACTCAACCCACGCTGCGGCCGCCGCCGCTGCCGCCGCTGCCTTCAAGCTGAGC
CCAGCCACCGCTCACGATCTGTCTTCGGGCCAGAGCTCAGCGTTCACACCGCAGGGTTCAGGTTATGCCA
ATGCCCTGGGCCACCATCACCACCACCATCACCACCATCACGCCAGCCAGGTGCCCACCTACGCGCGGCG
TGCCTCCGCCGCTTTCAACTCCACGCGCGACTTTCTGTTCCGTCAGCGCGGTTCTGGGCTCAGCGAGGCA
GCCTCCGGGGGCGGGCAGCACGGGCTTTTCGCTGGCTCGGCGAGCAGTCTTCACGCTCCAGCTGGTATTC
CTGAGCCTCCTAGCTACTTGCTCTTTCCTGGGCTTCATGAGCAGGGCGCTGGGCACCCGTCGCCCACAGG
GCACGTGGACAACAACCAGGTCCATCTGGGGCTGCGCGGGGAGCTATTTGGCCGTGCAGACCCATACCGC
CCCGTGGCTAGCCCGCGCACGGACCCCTACGCGGCCAGTGCGCAGTTCCCTAACTATAGCCCCATGAACA
TGAACATGGGCGTGAACGTGGCGGCCCACCACGGGCCGGGCGCCTTCTTCCGTTACATGCG
nt 646 - nt 1266 of NM_009575.1
Notes:The Zic3 probe used in this study by Elms et al., 2004 [PMID:15261827] was "a cDNA probe corresponding to bases 646-1266 of mouse Zic3 (Acc No.: NM_009575)".
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:involves: 101/HeH * C3H/HeH
Age:mid-streak Downs & Davies
Theiler Stage:TS10
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Elms P; Scurry A; Davies J; Willoughby C; Hacker T; Bogani D; Arkell R, 2004 [PMID:15261827] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.modgep.2004.03.003] [ PMID:15261827] Elms P, Scurry A, Davies J, Willoughby C, Hacker T, Bogani D, Arkell R 2004 Overlapping and distinct expression domains of Zic2 and Zic3 during mouse gastrulation. Gene Expr Patterns (4):505-11
Links:MGI:3526650 same experiment
 EMAGE:3980 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI