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EMAGE:3997

Hoxd13 homeobox D13 ( MGI:96205)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:3997 EMAGE:3997
Fig3B(left). Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2001.0382] Dev Biol 237: 345-53, Bruneau S; Johnson KR; Yamamoto M; Kuroiwa A; Duboule D, The mouse hoxd13(spdh) mutation, a polyalanine expansion similar to human type ii synpolydactyly (spd), disrupts the function but not the expression of other hoxd genes. Copyright 2001 Fig3C(left). Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2001.0382] Dev Biol 237: 345-53, Bruneau S; Johnson KR; Yamamoto M; Kuroiwa A; Duboule D, The mouse hoxd13(spdh) mutation, a polyalanine expansion similar to human type ii synpolydactyly (spd), disrupts the function but not the expression of other hoxd genes. Copyright 2001

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: Figure 3C is a higher magnification image of the forelimb of the embryo shown in figure 3B. In 3C anterior is left and posterior is right.
Expression Pattern Description
Spatial Annotation:
EMAGE:3997Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3997_wholemount_strong_3D_1.wlz
3997_wholemount_moderate_3D_1.wlz
3997_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3997_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
handplate
detected detected
Right forelimb autopod (Fig 3C).
embryo
detected detected
Fig 3B.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:12544
Entity Detected:Hoxd13, homeobox D13 ( MGI:96205)
Sequence:sense strand is shown

>MGI:12544
GCTGCCACGAACCTTTCGGAGAGACAAGTAACCATTTGGTTTCAGAATCGAAGGGTGAAGGACAAGAAAA
TCGTCTCCAAGCTCAAAGACACTGTCTCCTGATGTGGGCCAGGTTGGCCACAGACAGTTTAAATGCGTTC
AGTTGTCTCCAAAAAAATACCTTTGAAAAGACTTGAAAATGTATTTAATTTCCCTCCCCCTTCCCTGCCT
AGAAAGGCAAGCATTGTGAATCCTTTTCCCCCCTCTCTATCTCTCTCTCTCTCTCTTGACCTTTATCTGG
TTCTAAAACTTTTTGCTGCCCAATCCGACTTTGTAATTCTATTTCTTACTTGCTTATTATTGGTTTTGTT
CTTGTCTAGGTTTGTTTTTATAATTTTTAAACATTGTTTCTCCCTCCAGGCCAGTATGAGGAAAAAAAAA
CTTGAAGTATTTTTTTCAATAATCCCCCCAAATGAATTTCAGAAGTGCCCTTGTATTTAGGGGTTTATTT
TTTTAACAGGATTTTATATGTGTTTCCAGCACTGATTATGCTTATAATCCATTAGGCCAGAATCGTTTTT
TTTAGCCTTTCATACCCTGTGATTGTGTAATTGAATCATTAGCTCTCAGAAGTTTCCCCAAGGCAAGTGG
AAACAGCTCTTAGGCATTAGGTGATTTAGGACCGCTGGGAGAGTCTGAAACTTGGAGGTTGAAGGCACGT
GATGACTTTAAAAGCAGCTACCTTATTAATATTGAAATTAAGACAATCACCCTTATCTTTGGCAGTCTCC
TTTGCTGTTTTGTGTGGTTTTAACATGTCTTGTAGCTGTGATTTTTGAACCTGAAAACAGCATTCCTAGT
GGATGCCTTTATTTCTCCAACTCTACATGCAGCTATTAAAATACTTACACTTGGCAGAGTGACTGGAGAT
AGTATAAATTCATCCCGTTCCTTGGGTCTGTGGGAATGGAGCTAAGGCCATCTCCTCAATCCTTGGAAAG
AGACTCAGCCAATGTGGCCATGGGTGGGAACTTCTGTCCAGAGCTCAGTGAAGAAGTTAATTGTCTGAAT
GCATGGGCCTCTCAGAGCTTTAAAATATCTAACACATTTTTTAAATATGGAGCTCTAAGGACCAAGAGGG
AAAAAGAAACTTCTGAAAGTGATGAACTACTTTCAAAAATGTTGCGTATTTATGGGACTGAAAGATTATT
TTTGTAGATACAGTGTGTGTGAGCAGTGCTTCGAGCATTTAAAAATCACATTTTACTCCTAGGGAGATAT
AAATAAAAAGAACTCTCTTGTCTCCACTGAGTCTTCATCCATTTGTGTTTTTCA
nt 154967 - nt 156280 of AL928644.12
Notes:The Hoxd13 (Hox-4.8) probe used in this study by Bruneau et al., 2001 [PMID:11543619] is indicated as that used by Dolle et al, 1993 [PMID:8106170] , who in turn refer to Dolle et al, 1991 [PMID:1685889] , who define the probe as probeA (see Fig 1 therein), which is a "1.3kb PvuII-HindIII fragment" of mouse genomic DNA. Editors note: this probe contains some of the Hoxd13 homeobox sequence at its 5' end. The probe start and end co-ordinates with respect to the mouse Chr2 genomic sequence AL928644.12 were deduced using this information.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Bruneau S; Johnson KR; Yamamoto M; Kuroiwa A; Duboule D, 2001 [PMID:11543619] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2001.0382] [ PMID:11543619] Bruneau S, Johnson KR, Yamamoto M, Kuroiwa A, Duboule D 2001 The mouse Hoxd13(spdh) mutation, a polyalanine expansion similar to human type II synpolydactyly (SPD), disrupts the function but not the expression of other Hoxd genes. Dev Biol (237):345-53
 [ doi:10.1016/0092-8674(93)90378-4] [ PMID:8106170] Dolle P, Dierich A, LeMeur M, Schimmang T, Schuhbaur B, Chambon P, Duboule D 1993 Disruption of the Hoxd-13 gene induces localized heterochrony leading to mice with neotenic limbs. Cell (75):431-41
 [ doi:10.1016/0925-4773(91)90067-G] [ PMID:1685889] Dolle P, Izpisua-Belmonte JC, Boncinelli E, Duboule D 1991 The Hox-4.8 gene is localized at the 5' extremity of the Hox-4 complex and is expressed in the most posterior parts of the body during development. Mech Dev (36):3-13
Links:MGI:2153099 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI