Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:3998

Hoxd13 homeobox D13 ( MGI:96205)
TS19 (11.5 dpc)
immunohistochemistry

Data Images
EMAGE:3998 EMAGE:3998
Fig3F(left). Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2001.0382] Dev Biol 237: 345-53, Bruneau S; Johnson KR; Yamamoto M; Kuroiwa A; Duboule D, The mouse hoxd13(spdh) mutation, a polyalanine expansion similar to human type ii synpolydactyly (spd), disrupts the function but not the expression of other hoxd genes. Copyright 2001 Fig3G(left). Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2001.0382] Dev Biol 237: 345-53, Bruneau S; Johnson KR; Yamamoto M; Kuroiwa A; Duboule D, The mouse hoxd13(spdh) mutation, a polyalanine expansion similar to human type ii synpolydactyly (spd), disrupts the function but not the expression of other hoxd genes. Copyright 2001

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: wt - wild type. Figure 3G shows the forelimb (top) and hindlimb (bottom) of the embryo shown in figure 3F, at a higher magnification.
Expression Pattern Description
Spatial Annotation:
EMAGE:3998Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3998_wholemount_strong_3D_1.wlz
3998_wholemount_moderate_3D_1.wlz
3998_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3998_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
handplate ectoderm
not detected not detected
homogeneous
footplate ectoderm
not detected not detected
homogeneous
handplate mesenchyme
detected detected
regionalExpression is in the distal mesenchyme of the autopod.
footplate mesenchyme
detected detected
regionalExpression is in the distal mesenchyme of the autopod.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:2153105
Entity Detected:Hoxd13, homeobox D13 ( MGI:96205)
Notes:The anti-Hoxd13 antibody used in this study by Bruneau et al., 2001 [PMID:11543619] is described by the authors simply as being "a polyclonal antibody".
Antibody Type:polyclonal
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Bruneau S; Johnson KR; Yamamoto M; Kuroiwa A; Duboule D, 2001 [PMID:11543619] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2001.0382] [ PMID:11543619] Bruneau S, Johnson KR, Yamamoto M, Kuroiwa A, Duboule D 2001 The mouse Hoxd13(spdh) mutation, a polyalanine expansion similar to human type II synpolydactyly (SPD), disrupts the function but not the expression of other Hoxd genes. Dev Biol (237):345-53
Links:MGI:2153099 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI