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EMAGE:4011

Myf5 myogenic factor 5 ( MGI:97252)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4011 EMAGE:4011
Fig4Fleft. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1999.9215] Dev Biol 209: 143-58, Henderson DJ; Conway SJ; Copp AJ, Rib truncations and fusions in the Sp2H mouse reveal a role for Pax3 in specification of the ventro-lateral and posterior parts of the somite. Copyright 1999 Fig4G. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1999.9215] Dev Biol 209: 143-58, Henderson DJ; Conway SJ; Copp AJ, Rib truncations and fusions in the Sp2H mouse reveal a role for Pax3 in specification of the ventro-lateral and posterior parts of the somite. Copyright 1999

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
The apparent strong neural expression in this specimen is probably due to reagent trapping as the authors mention this problem in the legend of Figure 3, in reference to other samples. All samples in this paper show this problem to varying degrees.
Expression Pattern Description
Spatial Annotation:
EMAGE:4011Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4011_wholemount_strong_3D_1.wlz
4011_wholemount_moderate_3D_1.wlz
4011_wholemount_possible_3D_1.wlz
4011_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4011_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1335888
Entity Detected:Myf5, myogenic factor 5 ( MGI:97252)
Notes:The Myf5 probe template used in this study by Henderson et al., 1999 [PMID:10208749] was obtained from Simon Hughes (London) and is of unreported sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Henderson DJ; Conway SJ; Copp AJ, 1999 [PMID:10208749] , Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.1999.9215] [ PMID:10208749] Henderson DJ, Conway SJ, Copp AJ 1999 Rib truncations and fusions in the Sp2H mouse reveal a role for Pax3 in specification of the ventro-lateral and posterior parts of the somite. Dev Biol (209):143-58
Links:MGI:1336589 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI