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EMAGE:4012

Myf5 myogenic factor 5 ( MGI:97252)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4012
Fig3E. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2000.9788] Dev Biol 224: 29-41, Vivian JL; Olson EN; Klein WH, Thoracic skeletal defects in myogenin- and MRF4-deficient mice correlate with early defects in myotome and intercostal musculature. Copyright 2000

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4012Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4012_wholemount_strong_3D_1.wlz
4012_wholemount_moderate_3D_1.wlz
4012_wholemount_possible_3D_1.wlz
4012_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4012_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
regionalExpression in myotome.
tail somite
detected detected
regionalExpression in myotome.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1891641
Entity Detected:Myf5, myogenic factor 5 ( MGI:97252)
Notes:The Myf5 probe used in this study by Vivian et al., 2000 [PMID:10898959] is described as follows "a full-length mouse myf5 cDNA template was used to make a riboprobe (Braun et al., 1989 [PMID:2721498] ." Editors note: Braun et al describe the isolation of human myf5.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:either: C57BL/6 or (129S7/SvEvBrd x C57BL/6)F1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Vivian JL; Olson EN; Klein WH, 2000 [PMID:10898959] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2000.9788] [ PMID:10898959] Vivian JL, Olson EN, Klein WH 2000 Thoracic skeletal defects in myogenin- and MRF4-deficient mice correlate with early defects in myotome and intercostal musculature. Dev Biol (224):29-41
 [ PMID:2721498] Braun T, Buschhausen-Denker G, Bober E, Tannich E, Arnold HH 1989 A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts. EMBO J (8):701-9
Links:MGI:1913097 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI