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EMAGE:4014

Myf5 myogenic factor 5 ( MGI:97252)
TS19 (11.5 dpc)
in situ reporter

Data Images
EMAGE:4014
Fig5A. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0012-1606(03)00139-8] Dev Biol 258: 307-18, Kablar B; Krastel K; Tajbakhsh S; Rudnicki MA, Myf5 and MyoD activation define independent myogenic compartments during embryonic development. Copyright 2003

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4014Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4014_wholemount_strong_3D_1.wlz
4014_wholemount_moderate_3D_1.wlz
4014_wholemount_possible_3D_1.wlz
4014_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4014_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
regionalExpression is present in the myotome.
tail somite
detected detected
regionalExpression is present in the myotome.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ reporter
Entity Detected:Myf5, myogenic factor 5 ( MGI:97252)
Notes:Myf5 expression was detected in this study by Kablar et al., 2003 [PMID:12798290] using a Myf5-nlacZ reporter knock-in line, in which the nlacZ reporter gene is introduced into the Myf5 locus by homologous recombination (see Tajbakhsh et al., 1996 [PMID:8918877] for further details of this line's construction).
Labelled with:LacZ
Specimen
Organism:mouse
Strain:involves: 129S2/SvPas
Age:11.5 dpc
Theiler Stage:TS19
Mutations:Myf5tm1Pas/Myf5+
Preparation:wholemount
Procedures
Fixation:2% paraformaldehyde:0.2% gluteraldehyde
General Information
Authors:Kablar B; Krastel K; Tajbakhsh S; Rudnicki MA, 2003 [PMID:12798290] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0012-1606(03)00139-8] [ PMID:12798290] Kablar B, Krastel K, Tajbakhsh S, Rudnicki MA 2003 Myf5 and MyoD activation define independent myogenic compartments during embryonic development. Dev Biol (258):307-18
 [ doi:10.1038/384266a0] [ PMID:8918877] Tajbakhsh S, Rocancourt D, Buckingham M 1996 Muscle progenitor cells failing to respond to positional cues adopt non-myogenic fates in myf-5 null mice. Nature (384):266-70
Links:MGI:3531019 same experiment
 MGI:1857973 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI