EMAGE mouse gene expression spatial database

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EMAGE:4018

Myog myogenin ( MGI:97276)
TS17 (10.5 dpc)
in situ hybridisation

Data Images


Fig3A. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2000.9788] Dev Biol 224: 29-41, Vivian JL; Olson EN; Klein WH, Thoracic skeletal defects in myogenin- and MRF4-deficient mice correlate with early defects in myotome and intercostal musculature. Copyright 2000	Fig3C. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2000.9788] Dev Biol 224: 29-41, Vivian JL; Olson EN; Klein WH, Thoracic skeletal defects in myogenin- and MRF4-deficient mice correlate with early defects in myotome and intercostal musculature. Copyright 2000

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4018_wholemount_strong_3D_1.wlz

4018_wholemount_moderate_3D_1.wlz

4018_wholemount_possible_3D_1.wlz

4018_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4018_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trunk somite

detected

regional

Expression in myotome.

tail somite

detected

regional

Expression in myotome.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:1334560
Entity Detected:	Myog, myogenin ( MGI:97276)
Sequence:	sense strand is shown >MGI:1334560 CTGCGGGACGTTGGGGGCCAGTGGCAGGAACAAGCCTTTTGGCACCTGATGGAGCTGTATGAGACATCCC CCTATTTCTACCAGGAGCCCCACTTCTATGATGGGGAAAACTACCTTCCTGTCCACCTTCAGGGCTTCGA GCCCCCGGGCTATGAGCGGACTGAGCTCAGCTTAAGCCCGGAAGCCCGAGGGCCCCTGGAAGAAAAGGGA CTGGGGACCCCTGAGCATTGTCCAGGCCAGTGCCTGCCGTGGGCATGTAAGGTGTGTAAGAGGAAGTCTG TGTCGGTGGACCGGAGGAGGGCAGCCACACTGAGGGAGAAGCGCAGGCTCAAGAAAGTGAATGAGGCCTT CGAGGCCCTGAAGAGGAGCACCCTGCTCAACCCCAACCAGCGGCTGCCTAAAGTGGAGATCCTGCGCAGC GCCATCCAGTACATTGAGCGCCTACAGGCCTTGCTCAGCTCCCTCAACCAGGAGGAGCGCGATCTCCGCT ACAGAGGCGGGGGCGGGCCCAGCCCATGGTGCCCAGTGAATGCAACTCCCACAGCGCCTCCTGCAGTCCG GAGTGGGGCAATGCACTGGAGTTCGGTCCCAACCCAGGAGATCATTTGCTCGCGGCTGACCCTACAGACG CCCACAATCTGCACTCCCTTACGTCCATCGTGGACAGCATCACGGTGGAGGATATGTCTGTTGCCTTCCC AGACGAAACCATGCCCAACTGAGATTGTCTGTCAGGCTGGGTGTGCATGTGAGCCCCCAAGTTGGTGTCA AAAGCCATCACTTCTGTAGCAGGGGGCTTTTAAGTGGGGCTGTCCTGATGTCCAGAAAACAGCCCTGGGC TGCCACAAGCCAGACTCCCCACTCCCCATTCACATAAGGCTAACACCCAGCCCAGGAGGGAATTTAGCTG ACTCCTTAAAGCAGAGAGCATCCTCTTCTGAGGAGAGAAAGATGGAGTCCAGAGAGCCCCCTTGTTAATG TCCCTCAGTGGGGCAAACTCAGGAGCTTCTTTTTTGTTTATCATATTATGCCTCGAATTC
	nt 1 - nt 1040 of X15784.1
Notes:	The Myog (myogenin) probe used in this study by Vivian et al., 2000 [PMID:10898959] is described as "An EcoRI fragment of the mouse myogenin cDNA (Edmonson and Olson, 1989 [PMID:2473006] ), containing the 5' untranslated region and the entire coding region, but lacking most of the 3' untranslated region (corresponding to nucleotides +1 to +1040)". Editors note: X15784.1 is the mouse myogenin cDNA sequence submitted to GenBank by Edmonson and Olson, and matches the sequence of myogenin shown in Fig 1 of their paper [PMID:2473006] apart from a ~70nt sequence deletion at the 5' end. EcoRI cuts X15784.1 at position 1035. The 5' EcoRI site is presumed to be linker derived.
Chemistry:	RNA

Specimen

Organism:	mouse
Strain:	either: C57BL/6 or (129S7/SvEvBrd x C57BL/6)F1
Age:	10.5 dpc
Theiler Stage:	TS17
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:

paraformaldehyde

General Information

Authors:	Vivian JL; Olson EN; Klein WH, 2000 [PMID:10898959] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1006/dbio.2000.9788] [ PMID:10898959] Vivian JL, Olson EN, Klein WH 2000 Thoracic skeletal defects in myogenin- and MRF4-deficient mice correlate with early defects in myotome and intercostal musculature. Dev Biol (224):29-41
	[ doi:10.1101/gad.3.5.628] [ PMID:2473006] Edmondson DG, Olson EN 1989 A gene with homology to the myc similarity region of MyoD1 is expressed during myogenesis and is sufficient to activate the muscle differentiation program. Genes Dev (3):628-40
Links:	MGI:1913093 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source