EMAGE mouse gene expression spatial database

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EMAGE:4019

Myog myogenin ( MGI:97276)
TS19 (11.5 dpc)
in situ hybridisation

Data Images


Fig4A. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2000.9788] Dev Biol 224: 29-41, Vivian JL; Olson EN; Klein WH, Thoracic skeletal defects in myogenin- and MRF4-deficient mice correlate with early defects in myotome and intercostal musculature. Copyright 2000	Fig4C. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2000.9788] Dev Biol 224: 29-41, Vivian JL; Olson EN; Klein WH, Thoracic skeletal defects in myogenin- and MRF4-deficient mice correlate with early defects in myotome and intercostal musculature. Copyright 2000	Fig4E. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2000.9788] Dev Biol 224: 29-41, Vivian JL; Olson EN; Klein WH, Thoracic skeletal defects in myogenin- and MRF4-deficient mice correlate with early defects in myotome and intercostal musculature. Copyright 2000

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4019_wholemount_strong_3D_1.wlz

4019_wholemount_moderate_3D_1.wlz

4019_wholemount_possible_3D_1.wlz

4019_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4019_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trunk somite

detected

regional

Expression in skeletal muscle. Expression in tail myotome. Early intercostal musculature, intercostal muscles.

tail somite

detected

regional

Expression in skeletal muscle. Expression in tail myotome. Early intercostal musculature, intercostal muscles.

axial skeleton

not detected

homogeneous

Absent in presumptive cartilage between intercostal muscles.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:1334560
Entity Detected:	Myog, myogenin ( MGI:97276)
Sequence:	sense strand is shown >MGI:1334560 CTGCGGGACGTTGGGGGCCAGTGGCAGGAACAAGCCTTTTGGCACCTGATGGAGCTGTATGAGACATCCC CCTATTTCTACCAGGAGCCCCACTTCTATGATGGGGAAAACTACCTTCCTGTCCACCTTCAGGGCTTCGA GCCCCCGGGCTATGAGCGGACTGAGCTCAGCTTAAGCCCGGAAGCCCGAGGGCCCCTGGAAGAAAAGGGA CTGGGGACCCCTGAGCATTGTCCAGGCCAGTGCCTGCCGTGGGCATGTAAGGTGTGTAAGAGGAAGTCTG TGTCGGTGGACCGGAGGAGGGCAGCCACACTGAGGGAGAAGCGCAGGCTCAAGAAAGTGAATGAGGCCTT CGAGGCCCTGAAGAGGAGCACCCTGCTCAACCCCAACCAGCGGCTGCCTAAAGTGGAGATCCTGCGCAGC GCCATCCAGTACATTGAGCGCCTACAGGCCTTGCTCAGCTCCCTCAACCAGGAGGAGCGCGATCTCCGCT ACAGAGGCGGGGGCGGGCCCAGCCCATGGTGCCCAGTGAATGCAACTCCCACAGCGCCTCCTGCAGTCCG GAGTGGGGCAATGCACTGGAGTTCGGTCCCAACCCAGGAGATCATTTGCTCGCGGCTGACCCTACAGACG CCCACAATCTGCACTCCCTTACGTCCATCGTGGACAGCATCACGGTGGAGGATATGTCTGTTGCCTTCCC AGACGAAACCATGCCCAACTGAGATTGTCTGTCAGGCTGGGTGTGCATGTGAGCCCCCAAGTTGGTGTCA AAAGCCATCACTTCTGTAGCAGGGGGCTTTTAAGTGGGGCTGTCCTGATGTCCAGAAAACAGCCCTGGGC TGCCACAAGCCAGACTCCCCACTCCCCATTCACATAAGGCTAACACCCAGCCCAGGAGGGAATTTAGCTG ACTCCTTAAAGCAGAGAGCATCCTCTTCTGAGGAGAGAAAGATGGAGTCCAGAGAGCCCCCTTGTTAATG TCCCTCAGTGGGGCAAACTCAGGAGCTTCTTTTTTGTTTATCATATTATGCCTCGAATTC
	nt 1 - nt 1040 of X15784.1
Notes:	The Myog (myogenin) probe used in this study by Vivian et al., 2000 [PMID:10898959] is described as "An EcoRI fragment of the mouse myogenin cDNA (Edmonson and Olson, 1989 [PMID:2473006] ), containing the 5' untranslated region and the entire coding region, but lacking most of the 3' untranslated region (corresponding to nucleotides +1 to +1040)". Editors note: X15784.1 is the mouse myogenin cDNA sequence submitted to GenBank by Edmonson and Olson, and matches the sequence of myogenin shown in Fig 1 of their paper [PMID:2473006] apart from a ~70nt sequence deletion at the 5' end. EcoRI cuts X15784.1 at position 1035. The 5' EcoRI site is presumed to be linker derived.
Chemistry:	RNA

Specimen

Organism:	mouse
Strain:	either: C57BL/6 or (129S7/SvEvBrd x C57BL/6)F1
Age:	11.5 dpc
Theiler Stage:	TS19
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:

paraformaldehyde

General Information

Authors:	Vivian JL; Olson EN; Klein WH, 2000 [PMID:10898959] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1006/dbio.2000.9788] [ PMID:10898959] Vivian JL, Olson EN, Klein WH 2000 Thoracic skeletal defects in myogenin- and MRF4-deficient mice correlate with early defects in myotome and intercostal musculature. Dev Biol (224):29-41
	[ doi:10.1101/gad.3.5.628] [ PMID:2473006] Edmondson DG, Olson EN 1989 A gene with homology to the myc similarity region of MyoD1 is expressed during myogenesis and is sufficient to activate the muscle differentiation program. Genes Dev (3):628-40
Links:	MGI:1913093 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source