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EMAGE:4021

Nefm neurofilament, medium polypeptide ( MGI:97314)
TS17 (10.5 dpc)
immunohistochemistry

Data Images
EMAGE:4021 EMAGE:4021 EMAGE:4021 EMAGE:4021
Fig5C. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1999.9278] Dev Biol 210: 305-21, Nardelli J; Thiesson D; Fujiwara Y; Tsai FY; Orkin SH, Expression and genetic interaction of transcription factors GATA-2 and GATA-3 during development of the mouse central nervous system. Copyright 1999 Fig5A. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1999.9278] Dev Biol 210: 305-21, Nardelli J; Thiesson D; Fujiwara Y; Tsai FY; Orkin SH, Expression and genetic interaction of transcription factors GATA-2 and GATA-3 during development of the mouse central nervous system. Copyright 1999 Fig5E. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1999.9278] Dev Biol 210: 305-21, Nardelli J; Thiesson D; Fujiwara Y; Tsai FY; Orkin SH, Expression and genetic interaction of transcription factors GATA-2 and GATA-3 during development of the mouse central nervous system. Copyright 1999 Fig5G. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1999.9278] Dev Biol 210: 305-21, Nardelli J; Thiesson D; Fujiwara Y; Tsai FY; Orkin SH, Expression and genetic interaction of transcription factors GATA-2 and GATA-3 during development of the mouse central nervous system. Copyright 1999

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: black arrowhead - exit point of the trigeminal nerve; white arrowhead - exit point of facial/vestibuloacoustic nerve; III - common oculomotor nerve; IV - trochlear nerve; V - trigeminal nerve; VII/VIII - facial/vestibuloacoustic complex; IX - glossopharyngeal, X/XI - vagal/accessory ganglion complexes.
Expression Pattern Description
Spatial Annotation:
EMAGE:4021Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4021_wholemount_strong_3D_1.wlz
4021_wholemount_moderate_3D_1.wlz
4021_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4021_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
acoustic viii ganglion
detected detected
facial vii ganglion
detected detected
trigeminal v ganglion
detected detected
cranial ganglion
detected detected
Expression is detected in the common oculomotor (III) and trochlear (IV) nerves.
midbrain
detected detected
hindbrain
detected detected
Expression is detected in the rostral hindbrain.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1330766
Entity Detected:Nefm, neurofilament, medium polypeptide ( MGI:97314)
Notes:The neurofilament immunostaining carried out in this study by Nardelli et al., 1999 [PMID:10357893] utilised the 2H3 mouse monoclonal antibody (Development Studies, Hybridoma bank), directed against a 160-kDa neurofilament protein, used at the 1:5000 dilution as described previously (Schneider-Maunoury et al., 1993 [PMID:7903221] ).
Antibody Type:monoclonal
Raised In:mouse
Supplier:Developmental Studies Hybridoma Bank
Catalogue Number:2H3
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Nardelli J; Thiesson D; Fujiwara Y; Tsai FY; Orkin SH, 1999 [PMID:10357893] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.1999.9278] [ PMID:10357893] Nardelli J, Thiesson D, Fujiwara Y, Tsai FY, Orkin SH 1999 Expression and genetic interaction of transcription factors GATA-2 and GATA-3 during development of the mouse central nervous system. Dev Biol (210):305-21
 [ doi:10.1016/0092-8674(93)90329-O] [ PMID:7903221] Schneider-Maunoury S, Topilko P, Seitandou T, Levi G, Cohen-Tannoudji M, Pournin S, Babinet C, Charnay P 1993 Disruption of Krox-20 results in alteration of rhombomeres 3 and 5 in the developing hindbrain. Cell (75):1199-214
Links:MGI:1342736 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI