EMAGE mouse gene expression spatial database

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EMAGE:4039

Cux2 cut-like homeobox 2 ( MGI:107321)
TS18 (10.5 dpc)
in situ hybridisation

Data Images


Figure 1I. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S1567-133X(03)00123-6] Gene Expr Patterns 3: 571-7, Iulianella A; Vanden Heuvel G; Trainor P, Dynamic expression of murine Cux2 in craniofacial, limb, urogenital and neuronal primordia. Copyright 2003.

Expression pattern clarity:
Find spatially similar expression patterns:

Notes:

Note: Frontal section through the craniofacial region. ec, ectoderm; me, mesenchyme.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

3D mapping

spatial mapping

View mapped 3D expression image

Download individual expression domains:

4039_voxel_strong_3D_1.wlz

4039_voxel_moderate_3D_1.wlz

4039_voxel_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4039_all_domains.zip

Find spatially similar expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

1st branchial arch mandibular component

detected

regional

Expression is in the distal portion.

1st branchial arch mandibular component mesenchyme

detected

regional

Expression is in the distal portion.

1st branchial arch maxillary component

detected

regional

Expression is in the distal portion.

1st branchial arch mandibular component ectoderm

not detected

homogeneous

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:2687298
Entity Detected:	Cux2, cut-like homeobox 2 ( MGI:107321)
Sequence:	sense strand is shown >MGI:2687298 CCCTGGGTGGGAAGCCCTTCTCAAGCAGCCAGGCTGCGGGGGGCATCCAGGAGATGGTGGCCATGTCCCC AGAGCTGGACACATACTCCATCACCAAGAGAGTCAAGGAGGTCCTCACCGACAACAACCTAGGGCAGCGG CTGTTTGGTGAGAGCATCTTGGGGTTGACCCAGGGCTCCGTGTCAGATCTGCTGTCGAGGCCCAAGCCCT GGCACAAACTGAGCTTGAAGGGCCGGGAGCCCTTTGTGCGTATGCAGCTGTGGCTGAGTGACCCCCACAA CGTGGAGAAGCTTCGGGACATGAAGAAGCTGGAGAAGAAAGCCTATCTGAAGCGCCGCTATGGGCTCATC GGCACCGGCTCGGACAGCGAGTCACCGGCTGCGCACTCCGAGTGCCCCAGCCCGTGTTTGCAGCCCCAGG AGTTGAGTCTCATGCAGGCCAAGAAGCCCAGGGTGGTGCTGGCGCCCGCCGAGAAGGAGGCTCTGCGGAA GGCCTACCAGCTCGAGCCGTACCCCTCGCAGCAGACCATAGAGCTGCTCTCCTTCCAACTCAACCTCAAG ACGAACACCGTCATCAACTGGTTCCACAACTACAGGTCCAGGATGCGCCGTGAAATGCTGGTGGAGGGGA CACAGGATGATCCTGACTTTGACCCGAGTGGGGGTCCCAATGTCCTGACGCCAGGCCACACCCACAGAGA GCCCACCCCACAGAGCCCCGACTCAGAGACTGAGGACCAAAAGCCCCCCATGAAGAGCTTAGAGCTGCAA GAGCCTGAGGGTCCCCTACAGCGAGCTGCCCCAGACAGGGCTCTGGTGAAGATCAAACAGGAAGAGGGTT TGGAGGTGGATGGAGACAGTCAGCCCCAGGACGTGGGGGATCCAGACCGAGGGCAAGATGGCCCCAAAGA GGAGCATACCCACCCTCTGGGAAACAGTGACCTCTCAGAGCTGGCCCCAGGGCCCTTTCTTTCAGGCACA CCCAATCCAGACTGTCCTTCATTGCACAACCCCCAAGAAAAGGGGACTGGGGAACAGGTTCACTCAGAGC CTCTGAGTTTCAAGTCCACCTCCGAATCCTCCTGCTGCAGCCTGGAGGGGCCACCGAACTCTCCCTCTGT CATCTCCTCGCCAGACCTCACGACATGTGTGTCACCTGCCCCTTCCTCCTCAGCCCCCATCTCCCCATCC TTACCTGGTGCCCCACCTGCCAAAGTGCCGAGTACCAGCCCCACTGGTGACACAGCCGCAGCCTTGCACC CCAGCACTAAGGTGAACCCCAACTTGCAGCGGCGGCATGAGAAAATGGCCAACTTGAACAGTATAATCTA CCGGCTGGAGAGGGCTGCCAACCGGGAAGAGGTCCTGGAGTGGGAATTCTGAAGCTGGGGTTGAGGG
	nt 3174 - nt 4570 of NM_007804.1
Notes:	The Cux2 probe used in this study by Iulianella et al., 2003 [PMID:12971989] was "obtained by PCR amplification of E11.5 head RNA using the following primers: CCCTCAACCCCAGCTTCAG and CCCTGGGTGGGAAGCCCTT. The resulting 1.4 KB fragment contained the third cut domain and homeodomain up to 25 nucleotides following the stop site in the 3' untranslated region. The fragment was cloned into the pCRII-TOPO vector using the TOPO TA cloning kit from Invitrogen (Carlsbad, California) and confirmed by sequencing. Digoxigenin-labeled antisense riboprobes were transcribed using Sp6 RNA polymerase from EcoRV-linearized templates."
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD-1
Age:	10.5 dpc
Theiler Stage:	TS18
Mutations:	none (wild-type)
Preparation:	sectioned wholemount

Procedures

Fixation:	4% paraformaldehyde
Embedding:	paraffin

General Information

Authors:	Iulianella A; Vanden Heuvel G; Trainor P, 2003 [PMID:12971989] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/S1567-133X(03)00123-6] [ PMID:12971989] Iulianella A, Vanden Heuvel G, Trainor P 2003 Dynamic expression of murine Cux2 in craniofacial, limb, urogenital and neuronal primordia. Gene Expr Patterns (3):571-7
Links:	MGI:2687306 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source