EMAGE mouse gene expression spatial database

EMAGE:4047

Smad2 MAD homolog 2 (Drosophila) ( MGI:108051)
TS07 (5.5 dpc)
immunohistochemistry

Data Images


Figure 2A. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S1567-133X(03)00029-2] Gene Expr Patterns 3: 355-60, de Sousa Lopes SM; Carvalho RL; van den Driesche S; Goumans MJ; ten Dijke P; Mummery CL, Distribution of phosphorylated Smad2 identifies target tissues of TGF beta ligands in mouse development. Copyright 2003.

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Notes:

Note: Sagittal section. ee, embryonic ectoderm; en, (visceral endoderm); epc, ectoplacental cone; xe, extraembryonic ectoderm. Scale bar: 0.2mm.

Expression Pattern Description

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Structure

Level

Pattern

Notes

embryo

detected

single cell

Nuclear and/or cytoplasmic staining is random througout embryonic and extra-embryonic tissues.

extraembryonic component

detected

single cell

Nuclear and/or cytoplasmic staining is random througout embryonic and extra-embryonic tissues.

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EMAGE Editor

Detection Reagent

Type:	antibody
Identifier:	MGI:2673080
Entity Detected:	Smad2, MAD homolog 2 (Drosophila) ( MGI:108051)
Antigen:	sense strand is shown >MGI:2673080 SSMS
	aa 464 - aa 467 of NP_034884.2
Notes:	The anti-phospho-Smad2 antibody used in this study by de Sousa Lopes et al., 2003 [PMID:12799085] is indicated as that described by Persson et al., 1998 [PMID:9738456] i.e.: "For generation of antiserum PS2 recognising the phosphorylated Ctail in Smad2, a peptide KKK-SSpMSp, containing two C-terminal phosphoserine residues, was coupled to keyhole limpet hemocyanin (KLH) using glutaraldehyde, mixed with Freund's adjuvant, and used to immunise rabbits".
Antibody Type:	polyclonal
Raised In:	rabbit

Specimen

Procedures

Fixation:	2% paraformaldehyde
Embedding:	plastic
Secondary Antibody:	biotinylated goat anti-rabbit
Tertiary Antibody:	streptavidin-HRP
Labelled with:	horse radish peroxidase
Visualisation method:	DAB

General Information

Authors:	de Sousa Lopes SM; Carvalho RL; van den Driesche S; Goumans MJ; ten Dijke P; Mummery CL, 2003 [PMID:12799085] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/S1567-133X(03)00029-2] [ PMID:12799085] de Sousa Lopes SM, Carvalho RL, van den Driesche S, Goumans MJ, ten Dijke P, Mummery CL 2003 Distribution of phosphorylated Smad2 identifies target tissues of TGF beta ligands in mouse development. Gene Expr Patterns (3):355-60
	[ doi:10.1016/S0014-5793(98)00954-5] [ PMID:9738456] Persson U, Izumi H, Souchelnytskyi S, Itoh S, Grimsby S, Engstrom U, Heldin CH, Funa K, ten Dijke P 1998 The L45 loop in type I receptors for TGF-beta family members is a critical determinant in specifying Smad isoform activation. FEBS Lett (434):83-7
Links:	MGI:2673222 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source