EMAGE mouse gene expression spatial database

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EMAGE:4051

Smad2 MAD homolog 2 (Drosophila) ( MGI:108051)
TS13 (8.5 dpc)
immunohistochemistry

Data Images


Figure 3E. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S1567-133X(03)00029-2] Gene Expr Patterns 3: 355-60, de Sousa Lopes SM; Carvalho RL; van den Driesche S; Goumans MJ; ten Dijke P; Mummery CL, Distribution of phosphorylated Smad2 identifies target tissues of TGF beta ligands in mouse development. Copyright 2003.

Expression pattern clarity:
Find spatially similar expression patterns:

Notes:

Note: Transverse section. bw, body wall; da, dorsal aorta; fg, foregut; nc, notochord; nt, neural tube; s, somites; sv, sinus venosus of the heart. Scale bar: 0.2mm.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

3D mapping

spatial mapping

View mapped 3D expression image

Download individual expression domains:

4051_voxel_strong_3D_1.wlz

4051_voxel_moderate_3D_1.wlz

4051_voxel_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4051_all_domains.zip

Find spatially similar expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

heart

detected

foregut diverticulum

detected

trunk mesenchyme

detected

regional

Expression is in the body wall.

neural tube

detected

regional

Expression is in the rostral extremity of neural tube closure.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	antibody
Identifier:	MGI:2673080
Entity Detected:	Smad2, MAD homolog 2 (Drosophila) ( MGI:108051)
Antigen:	sense strand is shown >MGI:2673080 SSMS
	aa 464 - aa 467 of NP_034884.2
Notes:	The anti-phospho-Smad2 antibody used in this study by de Sousa Lopes et al., 2003 [PMID:12799085] is indicated as that described by Persson et al., 1998 [PMID:9738456] i.e.: "For generation of antiserum PS2 recognising the phosphorylated Ctail in Smad2, a peptide KKK-SSpMSp, containing two C-terminal phosphoserine residues, was coupled to keyhole limpet hemocyanin (KLH) using glutaraldehyde, mixed with Freund's adjuvant, and used to immunise rabbits".
Antibody Type:	polyclonal
Raised In:	rabbit

Specimen

Organism:	mouse
Strain:	involves: C57BL/6 * CBA
Age:	8.5 dpc
Theiler Stage:	TS13
Mutations:	none (wild-type)
Preparation:	sectioned wholemount

Procedures

Fixation:	2% paraformaldehyde
Embedding:	plastic
Secondary Antibody:	biotinylated goat anti-rabbit
Tertiary Antibody:	streptavidin-HRP
Labelled with:	horse radish peroxidase
Visualisation method:	DAB

General Information

Authors:	de Sousa Lopes SM; Carvalho RL; van den Driesche S; Goumans MJ; ten Dijke P; Mummery CL, 2003 [PMID:12799085] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/S1567-133X(03)00029-2] [ PMID:12799085] de Sousa Lopes SM, Carvalho RL, van den Driesche S, Goumans MJ, ten Dijke P, Mummery CL 2003 Distribution of phosphorylated Smad2 identifies target tissues of TGF beta ligands in mouse development. Gene Expr Patterns (3):355-60
	[ doi:10.1016/S0014-5793(98)00954-5] [ PMID:9738456] Persson U, Izumi H, Souchelnytskyi S, Itoh S, Grimsby S, Engstrom U, Heldin CH, Funa K, ten Dijke P 1998 The L45 loop in type I receptors for TGF-beta family members is a critical determinant in specifying Smad isoform activation. FEBS Lett (434):83-7
Links:	MGI:2673222 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source