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EMAGE:4057

Robo3 roundabout homolog 3 (Drosophila) ( MGI:1343102)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4057 EMAGE:4057
Figure 1C. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S1567-133X(03)00142-X] Gene Expr Patterns 4: 99-103, Camurri L; Mambetisaeva E; Sundaresan V, Rig-1 a new member of Robo family genes exhibits distinct pattern of expression during mouse development. Copyright 2004. Figure 1D. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S1567-133X(03)00142-X] Gene Expr Patterns 4: 99-103, Camurri L; Mambetisaeva E; Sundaresan V, Rig-1 a new member of Robo family genes exhibits distinct pattern of expression during mouse development. Copyright 2004.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: The arrows indicate the anterior part of the midbrain (C,D). hb, hindbrain; sc, spinal cord.
Expression Pattern Description
Spatial Annotation:
EMAGE:4057Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4057_wholemount_strong_3D_1.wlz
4057_wholemount_moderate_3D_1.wlz
4057_wholemount_weak_3D_1.wlz
4057_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4057_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future midbrain
detected detected
regionalExpression is in parallel stripes from the caudal part of the midbrain.
future hindbrain
detected detected
regionalExpression is in an irregular stripe with a gap just before the spinal cord.
future spinal cord
detected detected
regionalExpression is in a rostro-caudal direction in both sides of the spinal cord.
future midbrain roof plate
detected detected
regionalExpression is in bilateral spots.
future forebrain
not detected not detected
homogeneous
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3026875
Entity Detected:Robo3, roundabout homolog 3 (Drosophila) ( MGI:1343102)
Notes:The Robo3 probe used in this study by Camurri et al., 2004 [PMID:14678835] was generated by the following means: "Rig-1 [Robo3] cDNA sequence was obtained from NCBI database (GenBank accession number AF060570) and used to identify the mouse ESTs IMAGE 10336-f21, encoding for a 2 kb probe". Editor's note: unable to identify IMAGE clone 10336-f21.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Camurri L; Mambetisaeva E; Sundaresan V, 2004 [PMID:14678835] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S1567-133X(03)00142-X] [ PMID:14678835] Camurri L, Mambetisaeva E, Sundaresan V 2004 Rig-1 a new member of Robo family genes exhibits distinct pattern of expression during mouse development. Gene Expr Patterns (4):99-103
Links:MGI:3026881 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI