EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:4063

Ran RAN, member RAS oncogene family ( MGI:1333112)
TS15 (9.0 dpc)
in situ hybridisation

Data Images


Fig2C. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(02)00007-2] Mech Dev 113: 103-6, Lopez-Casas PP; Lopez-Fernandez LA; Krimer DB; del Mazo J, Ran GTPase expression during early development of the mouse embryo. Copyright 2002

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Image annotations: 1B - first branchial arch (mandibular component); Fb - forebrain; Hb - hindbrain; Mb - midbrain; Otv - otic vesicle; Ov - optic vesicle; Pnp - posterior neuropore.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4063_wholemount_strong_3D_1.wlz

4063_wholemount_moderate_3D_1.wlz

4063_wholemount_weak_3D_1.wlz

4063_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4063_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

caudal neuropore

detected

optic vesicle

detected

1st branchial arch mandibular component

detected

1st branchial arch maxillary component

detected

2nd branchial arch

detected

inner ear

detected

regional

Expression is in the otic vesicle.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:3029296
Entity Detected:	Ran, RAN, member RAS oncogene family ( MGI:1333112)
Sequence:	sense strand is shown >MGI:3029296 GGATCCGCCGCGATGGCCGCCCAGGGAGAGCCGCAGGTCCAGTTCAAGCTCGTCCTGGTGGGCGACGGCG GCACCGGGAAGACAACCTTCGTGAAGCGCCACTTGACGGGCGAGTTTGAGAAGAAGTATGTAGCCACCCT GGGCGTGGAGGTGCACCCGCTCGTCTTCCATACCAACAGAGGACCCATCAAGTTCAACGTGTGGGACACG GCCGGCCAGGAGAAGTTCGGGGGCCTGCGCGATGGCTACTACATCCAAGCCCAGTGTGCCATTATAATGT TTGATGTAACCTCAAGAGTTACTTACAAGAATGTACCTAACTGGCATAGAGATCTGGTACGAGTGTGTGA AAACATCCCCATTGTATTGTGTGGCAACAAAGTGGATATTAAAGACAGGAAAGTGAAGGCAAAATCTATT GTCTTCCACCGGAAGAAGAATCTTCAGTACTATGACATTTCTGCCAAAAGTAACTACAACTTTGAAAAGC CTTTCCTCTGGCTTGCCAGAAAGCTCATTGGAGATCCTAACTTGGAGTTTGTTGCCATGCCTGCTCTTGC CCCACCTGAGGTGGTCATGGACCCAGCTTTGGCAGCACAGTACGAGCATGATTTAGAGGTTGCTCAGACG ACTGCTCTCCCAGATGAGGATGATGACCTGTGAGAAAGTGAAGCTGGAGCC
	nt 14 - nt 694 of L32751.1
Notes:	The Ran probe used in this study by Lopez-Casas et al, 2002 [PMID:11900983] is described as corresponding "to a BamHI/BanII (676 bp) fragment subcloned from mouse Ran/M1 cDNA (Coutavas et al., 1994 [PMID:7849398] )". Editors Note: L32751.1 is the sequence submitted to GenBank for clone M1 of mouse Ran cDNA by Coutavas et al and the start and end co-ordinates w.r.t. to L32751.1 were deduced using this information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD-1
Age:	9.0 dpc
Theiler Stage:	TS15
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:

4% paraformaldehyde

General Information

Authors:	Lopez-Casas PP; Lopez-Fernandez LA; Krimer DB; del Mazo J, 2002 [PMID:11900983] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/S0925-4773(02)00007-2] [ PMID:11900983] Lopez-Casas PP, Lopez-Fernandez LA, Krimer DB, del Mazo J 2002 Ran GTPase expression during early development of the mouse embryo. Mech Dev (113):103-6
	[ doi:10.1007/BF00411457] [ PMID:7849398] Coutavas EE, Hsieh CM, Ren M, Drivas GT, Rush MG, D'Eustachio PD 1994 Tissue-specific expression of Ran isoforms in the mouse. Mamm Genome (5):623-8
Links:	MGI:3029300 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source