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EMAGE:4064

Ran RAN, member RAS oncogene family ( MGI:1333112)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4064 EMAGE:4064
Fig2D1. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(02)00007-2] Mech Dev 113: 103-6, Lopez-Casas PP; Lopez-Fernandez LA; Krimer DB; del Mazo J, Ran GTPase expression during early development of the mouse embryo. Copyright 2002 Fig2D. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(02)00007-2] Mech Dev 113: 103-6, Lopez-Casas PP; Lopez-Fernandez LA; Krimer DB; del Mazo J, Ran GTPase expression during early development of the mouse embryo. Copyright 2002

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Fig2D is a dorsal view of the embryo shown in Fig2D1. Image annotations: 1B - first branchial arch (mandibular component); 1Bx - first branchial arch (maxillary component); 2B - second branchial arch; Cnt - caudal neural tube; Flb - forelimb bud; Hb - hindbrain; Hbw - hindbrain wall; Mb - midbrain; Mbw - midbrain wall; Otv - otic vesicle; Ov - optic vesicle; Tv - telencephalic vesicle.
Expression Pattern Description
Spatial Annotation:
EMAGE:4064Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4064_wholemount_strong_3D_1.wlz
4064_wholemount_moderate_3D_1.wlz
4064_wholemount_weak_3D_1.wlz
4064_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4064_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
optic vesicle
detected detected
otocyst
detected detected
1st branchial arch mandibular component
detected detected
1st branchial arch maxillary component
detected detected
2nd branchial arch
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3029296
Entity Detected:Ran, RAN, member RAS oncogene family ( MGI:1333112)
Sequence:sense strand is shown

>MGI:3029296
GGATCCGCCGCGATGGCCGCCCAGGGAGAGCCGCAGGTCCAGTTCAAGCTCGTCCTGGTGGGCGACGGCG
GCACCGGGAAGACAACCTTCGTGAAGCGCCACTTGACGGGCGAGTTTGAGAAGAAGTATGTAGCCACCCT
GGGCGTGGAGGTGCACCCGCTCGTCTTCCATACCAACAGAGGACCCATCAAGTTCAACGTGTGGGACACG
GCCGGCCAGGAGAAGTTCGGGGGCCTGCGCGATGGCTACTACATCCAAGCCCAGTGTGCCATTATAATGT
TTGATGTAACCTCAAGAGTTACTTACAAGAATGTACCTAACTGGCATAGAGATCTGGTACGAGTGTGTGA
AAACATCCCCATTGTATTGTGTGGCAACAAAGTGGATATTAAAGACAGGAAAGTGAAGGCAAAATCTATT
GTCTTCCACCGGAAGAAGAATCTTCAGTACTATGACATTTCTGCCAAAAGTAACTACAACTTTGAAAAGC
CTTTCCTCTGGCTTGCCAGAAAGCTCATTGGAGATCCTAACTTGGAGTTTGTTGCCATGCCTGCTCTTGC
CCCACCTGAGGTGGTCATGGACCCAGCTTTGGCAGCACAGTACGAGCATGATTTAGAGGTTGCTCAGACG
ACTGCTCTCCCAGATGAGGATGATGACCTGTGAGAAAGTGAAGCTGGAGCC
nt 14 - nt 694 of L32751.1
Notes:The Ran probe used in this study by Lopez-Casas et al, 2002 [PMID:11900983] is described as corresponding "to a BamHI/BanII (676 bp) fragment subcloned from mouse Ran/M1 cDNA (Coutavas et al., 1994 [PMID:7849398] )". Editors Note: L32751.1 is the sequence submitted to GenBank for clone M1 of mouse Ran cDNA by Coutavas et al and the start and end co-ordinates w.r.t. to L32751.1 were deduced using this information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Lopez-Casas PP; Lopez-Fernandez LA; Krimer DB; del Mazo J, 2002 [PMID:11900983] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1007/BF00411457] [ PMID:7849398] Coutavas EE, Hsieh CM, Ren M, Drivas GT, Rush MG, D'Eustachio PD 1994 Tissue-specific expression of Ran isoforms in the mouse. Mamm Genome (5):623-8
 [ doi:10.1016/S0925-4773(02)00007-2] [ PMID:11900983] Lopez-Casas PP, Lopez-Fernandez LA, Krimer DB, del Mazo J 2002 Ran GTPase expression during early development of the mouse embryo. Mech Dev (113):103-6
Links:MGI:3029300 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI