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EMAGE:4066

Pdgfa platelet derived growth factor, alpha ( MGI:97527)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4066
Fig6A(left). Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1999.9215] Dev Biol 209: 143-58, Henderson DJ; Conway SJ; Copp AJ, Rib truncations and fusions in the Sp2H mouse reveal a role for Pax3 in specification of the ventro-lateral and posterior parts of the somite. Copyright 1999

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: arrows indicate Pdgfa expression in a segmental pattern in the flank of the embryo.
Expression Pattern Description
Spatial Annotation:
EMAGE:4066Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4066_wholemount_strong_3D_1.wlz
4066_wholemount_moderate_3D_1.wlz
4066_wholemount_weak_3D_1.wlz
4066_wholemount_possible_3D_1.wlz
4066_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4066_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
regionalExpression is seen in a segmental pattern in the flank.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1335890
Entity Detected:Pdgfa, platelet derived growth factor, alpha ( MGI:97527)
Notes:The Pdgfa probe used in this study by Henderson et al., 1999 [PMID:10208749] is described as a gift from Mark Mercola, Boston.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Henderson DJ; Conway SJ; Copp AJ, 1999 [PMID:10208749] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.1999.9215] [ PMID:10208749] Henderson DJ, Conway SJ, Copp AJ 1999 Rib truncations and fusions in the Sp2H mouse reveal a role for Pax3 in specification of the ventro-lateral and posterior parts of the somite. Dev Biol (209):143-58
Links:MGI:1346769 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI