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EMAGE:4069

Pdgfra platelet derived growth factor receptor, alpha polypeptide ( MGI:97530)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:4069 EMAGE:4069
Fig6F(left). Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1999.9215] Dev Biol 209: 143-58, Henderson DJ; Conway SJ; Copp AJ, Rib truncations and fusions in the Sp2H mouse reveal a role for Pax3 in specification of the ventro-lateral and posterior parts of the somite. Copyright 1999 Fig6G. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1999.9215] Dev Biol 209: 143-58, Henderson DJ; Conway SJ; Copp AJ, Rib truncations and fusions in the Sp2H mouse reveal a role for Pax3 in specification of the ventro-lateral and posterior parts of the somite. Copyright 1999

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Fig6G is a section taken from the embryo shown in Fig6F. Image annotations: Fig6F arrows indicate expression of Pdgfra in the posterior part of each somite. Fig6G small arrow indicates expression in the dermomyotome, large arrow indicates expression in the dorsal mesoderm, arrowhead indicates lack of expression in the myotome, nt - neural tube, drg - dorsal root ganglia.
Expression Pattern Description
Spatial Annotation:
EMAGE:4069Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4069_wholemount_strong_3D_1.wlz
4069_wholemount_moderate_3D_1.wlz
4069_wholemount_weak_3D_1.wlz
4069_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4069_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
regionalExpression is restricted to the posterior part of each somite. The myotome no longer expresses at this stage.
embryo
detected detected
regionalExpression is detected in the dorsal mesoderm.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1335891
Entity Detected:Pdgfra, platelet derived growth factor receptor, alpha polypeptide ( MGI:97530)
Notes:The Pdgfra probe used in this study by Henderson et al., 1999 [PMID:10208749] is described as a gift from Mark Mercola, Boston.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Henderson DJ; Conway SJ; Copp AJ, 1999 [PMID:10208749] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.1999.9215] [ PMID:10208749] Henderson DJ, Conway SJ, Copp AJ 1999 Rib truncations and fusions in the Sp2H mouse reveal a role for Pax3 in specification of the ventro-lateral and posterior parts of the somite. Dev Biol (209):143-58
Links:MGI:1346770 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI