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EMAGE:4071

Sox14 SRY-box containing gene 14 ( MGI:98362)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:4071 EMAGE:4071
Fig2B. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1999.9581] Dev Biol 219: 142-53, Hargrave M; Karunaratne A; Cox L; Wood S; Koopman P; Yamada T, The HMG box transcription factor gene Sox14 marks a novel subset of ventral interneurons and is regulated by sonic hedgehog. Copyright 2000 Fig2D. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1999.9581] Dev Biol 219: 142-53, Hargrave M; Karunaratne A; Cox L; Wood S; Koopman P; Yamada T, The HMG box transcription factor gene Sox14 marks a novel subset of ventral interneurons and is regulated by sonic hedgehog. Copyright 2000

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Fig2D is a hemisected embryo head of the same stage as that shown in Fig2B. In D: Expression domains in the pretectum (arrow), in the ventral diencephalon (arrowhead), and adjacent to the zona limitans intrathalamica (zl, dotted line).
Expression Pattern Description
Spatial Annotation:
EMAGE:4071Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4071_wholemount_strong_3D_1.wlz
4071_wholemount_moderate_3D_1.wlz
4071_wholemount_weak_3D_1.wlz
4071_wholemount_possible_3D_1.wlz
4071_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4071_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
brain
detected detected
future spinal cord
detected detected
regionalExpression detected in the rostral spinal cord, tectum, pretectum and ventral diencephalon.
midbrain
detected detected
regionalExpression detected in the rostral spinal cord, tectum, pretectum and ventral diencephalon.
hindbrain
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1859734
Entity Detected:Sox14, SRY-box containing gene 14 ( MGI:98362)
Sequence:sense strand is shown

>MGI:1859734
CGGCCGGCCTGCCCGTGGGGGCTTCCGACGGCCTCCTGAGCGCGCCCGAGAAAGCCCGGGCCTTCTTGCC
GCCTGCCTCGGCACCTTACTCCCTGCTGGACCCTGCGCAATTTAGTTCCAGTGCCATCCAGAAGATGGGT
GAAGTGCCCCACACGTTGGCCACCAGCGCGCTGCCCTACGCGTCCACCCTGGGCTATCAGAACGGCGCCT
TCGGCAGTCTCAGCTGCCCCAGCCAGCACACGCACACGCACCCGTCCCCCACCAACCCGGGCTACGTGGT
GCCCTGTAACTGTACCGCCTGGTCTGCCTCCACCCTGCAGCCCCCTGTCGCCTACATCCTCTTCCCAGGC
ATGACCAAGACTGGCATAGACCCTTATTCGTCAGCCCACGCTACGGCCATGTAACCCCA
nt 305 - nt 713 of AF193435.1
Notes:The Sox14 probe template used in this study by Hargrave et al., 2000 [PMID:10677261] is described as "EagI-HindIII fragment, 414bp." The GenBank ID of the cDNA sequence (AF193435) is also given in the paper.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Hargrave M; Karunaratne A; Cox L; Wood S; Koopman P; Yamada T, 2000 [PMID:10677261] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.1999.9581] [ PMID:10677261] Hargrave M, Karunaratne A, Cox L, Wood S, Koopman P, Yamada T 2000 The HMG box transcription factor gene Sox14 marks a novel subset of ventral interneurons and is regulated by sonic hedgehog. Dev Biol (219):142-53
Links:MGI:1859939 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI