Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:4073

Tbx6 T-box 6 ( MGI:102539)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4073
Fig2H. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1996.0326] Dev Biol 180: 534-42, Chapman DL; Agulnik I; Hancock S; Silver LM; Papaioannou VE, Tbx6, a mouse T-Box gene implicated in paraxial mesoderm formation at gastrulation. Copyright 1996

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4073Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4073_wholemount_strong_3D_1.wlz
4073_wholemount_moderate_3D_1.wlz
4073_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4073_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
tail unsegmented mesenchyme
detected detected
regionalExpression is in the caudal region extending from the area of the open neural tube rostrally to the level of neural tube closure.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:33996
Entity Detected:Tbx6, T-box 6 ( MGI:102539)
Notes:The Tbx6 probe used in this study by Chapman et al., 1996 [PMID:8954725] is described as the linearised plasmid w32. w32 is described as containing approximately 1150 bp of the Tbx6 cDNA, including part of the T-box region, inserted at the EcoRI site of pBluescript II KS(-). The probe was hydrolyzed to an average size of 0.5 kb for whole-mount in situ analysis.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:Swiss Webster
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Chapman DL; Agulnik I; Hancock S; Silver LM; Papaioannou VE, 1996 [PMID:8954725] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.1996.0326] [ PMID:8954725] Chapman DL, Agulnik I, Hancock S, Silver LM, Papaioannou VE 1996 Tbx6, a mouse T-Box gene implicated in paraxial mesoderm formation at gastrulation. Dev Biol (180):534-42
Links:MGI:1340265 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI