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EMAGE:4079

Tgfb1i1 transforming growth factor beta 1 induced transcript 1 ( MGI:102784)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4079
Fig7B. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2001.0313] Dev Biol 235: 507-20, Brunskill EW; Witte DP; Yutzey KE; Potter SS, Novel cell lines promote the discovery of genes involved in early heart development. Copyright 2001

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: Arrow points to heart; arrowheads point to somites. A - atria; V - ventricle.
Expression Pattern Description
Spatial Annotation:
EMAGE:4079Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4079_wholemount_strong_3D_1.wlz
4079_wholemount_moderate_3D_1.wlz
4079_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4079_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
heart atrium
detected detected
primitive ventricle
detected detected
trunk somite
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2150360
Entity Detected:Tgfb1i1, transforming growth factor beta 1 induced transcript 1 ( MGI:102784)
Sequence:sense strand is shown

>MGI:2150360
CACGGTTAGGGGCTCCAAAAGAGCGCCCACCAGAGACACTCACACCTCCCCCACCCTATGGCCACCAGCC
ACAGACAGGGTCTGGAGAATCTTCAGGAACCACTGGGGACAAGGATCATCTATACAGCACAGTATGCAAG
CCTCGGTCCCCAAAGCCTGTGGCCCCTGTGGCTCCTCCATTCTCCTCTTCCAGTGGTGTGTTGGGCAATG
GCCTCTGTGAGCTAGACCGTTTGCTTCAGGAACTTAATGCCACCCAGTTCAACATTACAGATGAAATCAT
GTCTCAGTTCCCATCTAGTAAAATGGCTGAAGGGGAAGAGAAGGAGGACCAATCTGAAGACAAGAGCTCA
CCCACTGTCCCTCCCAGCCCATTCCCTGCCCCCTCAAAGCCTTCAGCCACCTCCGCCACTCAGGAACTGG
ATAGACTGATGGCCTCGCTCTCTGACTTCCGTGTTCAGAACCATCTTCCAGCCTCAGGGCCACCTCAGCC
TCCAGCAGCGAGCCCCACCCGTGAAGGATGCCCATCTCCACCAGGACAGACTAGCAAAGGCAGTCTGGAC
ACCATGCTGGGCCTGCTGCAGTCTGACCTCAGCCGTCGTGGTGTCCCCACACAGGCCAAAGGCCTCTGTG
GCTCCTGCAATAAACCTATAGCTGGGCAAG
nt 201 - nt 860 of L22482.1
Notes:The Tgfb1i1 (Hic-5) probe used in this study by Brunskill et al., 2001 [PMID:11437454] is described as follows:" The Hic-5 probe was generated by RT-PCR using RNA isolated from the 1H cellline and the following oligonucleotides: Hic-5, 5'-CACGGTTAGGGGCTCCAAAA and 5'-CTTGCCCAGCTATAGGTTTA." The cell line 1H was derived from three E10.5 embryonic hearts - description in paper.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Brunskill EW; Witte DP; Yutzey KE; Potter SS, 2001 [PMID:11437454] , , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2001.0313] [ PMID:11437454] Brunskill EW, Witte DP, Yutzey KE, Potter SS 2001 Novel cell lines promote the discovery of genes involved in early heart development. Dev Biol (235):507-20
Links:MGI:2150376 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI