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EMAGE:4088

Cdh6 cadherin 6 ( MGI:107435)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4088
Fig6C. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2001.0447] Dev Biol 240: 15-31, Watari N; Kameda Y; Takeichi M; Chisaka O, Hoxa3 regulates integration of glossopharyngeal nerve precursor cells. Copyright 2001

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: The arrowheads indicate the positions of the proximal and distal ganglion anlagen of the IXth nerve. The arrows indicate the neural crest cells associated with the nerve.
Expression Pattern Description
Spatial Annotation:
EMAGE:4088Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4088_wholemount_strong_3D_1.wlz
4088_wholemount_moderate_3D_1.wlz
4088_wholemount_weak_3D_1.wlz
4088_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4088_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
head mesenchyme derived from neural crest
detected detected
regionalExpression is from the proximal ganglion to the distal ganglion anlagen of the IXth nerve.
glossopharyngeal ix preganglion
detected detected
regionalExpression in neural crest cells associated with the IXth nerve
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1859640
Entity Detected:Cdh6, cadherin 6 ( MGI:107435)
Sequence:sense strand is shown

>MGI:1859640
GGATCCGATTATCAGTACGTGGGCAAGTTACATTCAGACCAGGATAGAGGAGATGGATCACTTAAATATA
TCCTTTCAGGAGATGGAGCGGGCGATCTCTTCATTATCAATGAGCACACAGGCGACATACAGGCCACCAA
GAGGCTGGACAGGGAAGAAAAACCTGTTTACATCCTTCGAGCTCAAGCTGTCAACAGAAGGACAGGGAGA
CCCGTCGAGCCTGAGTCTGAATTCATCATCAAGATCCACGACATCAATGACAATGAGCCAATATTCACCA
AGGACGTTTACACAGCCACAGTCCCGGAGATGGCTGATGTTGGCACATTTGTGGTCCAAGTCACGGCGAC
TGATGCTGACGACCCAACATATGGGAACAGTGCTAAAGTCGTCTATAGCATCCTGCAGGGACAGCCCTAC
TTTTCAGTGGAATCGGAAACAGGTATCATCAAGACAGCGTTGCTCAACATGGATCGAGAAAACAGAGAAC
AGTACCAAGTGGTGATTCAGGCCAAGGACATGGGCGGCCAGATGGGAGGACTGTCGGGGACTACGACCGT
GAACATCACGCTGACAGATGTTAATGACAACCCACCCCGTTTCCCCCAGAGTACCTACCAGTTTAAGACC
CCCGAGTCCTCTCCACCGGGAACGCCAATTGGCAGGATCAAAGCCAGTGATGCCGATGTGGGAGAAAATG
CGGAGATCGAGTACAGCATCACTGACGGTGAGGGACACGAGATGTTTGATGTCATCACCGACCAGGAAAC
CCAGGAAGGGATCATAACTGTCAAAAAGCTCTTGGATTTTGAGAAGAAGAAGGTGTACACCCTCAGGTGG
AAGGCCTCCAATCCCCACGTCGAACCCCGATTTCTCTACCTGGGTCCCTTCAAAGACTCGGCCACGGTGA
GAATCGTGGTGGACGATGTGGACGAACCTCCTGTCTTCAGCAAACTGGCCTACATCCTACAGATACGGGA
GGATGCCCGGATAAATACGACCATAGTCTCTGTCGCAGCTCAGGATCC
nt 202 - nt 1229 of D82029.1
Notes:The Cdh6 (cadherin6) probe used in this study by Watari et al., 2001 [PMID:11784044] was previously described by Inoue et al., 1997 [PMID:9126293] as follows: "Sense or antisense cad6 RNA probes labeled with digoxigenin (Dig)-11-UTP (Boehringer-Mannheim) were prepared from pBSII1.0B, which contains the 202-1229 BamHI fragment of cad6 cDNA. For the antisense probe, the EcoRI linearized vector was transcribed with T3 RNA polymerase (Gibco BRL)." Editors note: D82029.1 is the Cdh6 sequence submitted to GenBank by Inoue. It was confirmed that BamHI cuts this sequence at positions 202 and 1229.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Watari N; Kameda Y; Takeichi M; Chisaka O, 2001 [PMID:11784044] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2001.0447] [ PMID:11784044] Watari N, Kameda Y, Takeichi M, Chisaka O 2001 Hoxa3 regulates integration of glossopharyngeal nerve precursor cells. Dev Biol (240):15-31
 [ doi:10.1006/dbio.1996.8501] [ PMID:9126293] Inoue T, Chisaka O, Matsunami H, Takeichi M 1997 Cadherin-6 expression transiently delineates specific rhombomeres, other neural tube subdivisions, and neural crest subpopulations in mouse embryos. Dev Biol (183):183-94
Links:MGI:2156499 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI