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EMAGE:4091

Pitx2 paired-like homeodomain transcription factor 2 ( MGI:109340)
TS16 (10.0 dpc)
in situ hybridisation

Data Images
EMAGE:4091 EMAGE:4091
Fig5A. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2000.0133] Dev Biol 231: 252-64, Campione M; Ros MA; Icardo JM; Piedra E; Christoffels VM; Schweickert A; Blum M; Franco D; Moorman AF, Pitx2 expression defines a left cardiac lineage of cells: evidence for atrial and ventricular molecular isomerism in the iv/iv mice. Copyright 2001 Fig5C. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2000.0133] Dev Biol 231: 252-64, Campione M; Ros MA; Icardo JM; Piedra E; Christoffels VM; Schweickert A; Blum M; Franco D; Moorman AF, Pitx2 expression defines a left cardiac lineage of cells: evidence for atrial and ventricular molecular isomerism in the iv/iv mice. Copyright 2001

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Fig5C is a ventral view of the embryonic heart shown in a lateral view in Fig5A. Image annotations: The body A-P and D-V axes are indicated. The black arrow indicates expression. la - left atrium; avc - atrioventricular canal; lv - left ventricle; oft - outflow tract; rv - right ventricle; ra - right atrium.
Expression Pattern Description
Spatial Annotation:
EMAGE:4091Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4091_wholemount_strong_3D_1.wlz
4091_wholemount_moderate_3D_1.wlz
4091_wholemount_weak_3D_1.wlz
4091_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4091_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
common atrial chamber left part
detected detected
outflow tract
detected detected
regionalExpression is present in the left/ventral portion of the outflow tract, as well as in the mesenchyme that will form the aortic sac.
primitive ventricle
detected detected
regionalExpression is present in the ventral portion of the left ventricle, beside the interventricular groove.
atrioventricular canal
detected detected
regionalExpression is present in the anterior part.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1330045
Entity Detected:Pitx2, paired-like homeodomain transcription factor 2 ( MGI:109340)
Notes:The Pitx2 probe used in this study by Campione et al., 2001 [PMID:11180966] was previously described by Campione et al., 1999 [PMID:10021341] as follows: mouse Pitx2 (1.7 kb XhoI-EcoRI fragment corresponding to entire coding sequence plus 5' and 3' UTR).
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:FVB
Age:10.0 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Campione M; Ros MA; Icardo JM; Piedra E; Christoffels VM; Schweickert A; Blum M; Franco D; Moorman AF, 2001 [PMID:11180966] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2000.0133] [ PMID:11180966] Campione M, Ros MA, Icardo JM, Piedra E, Christoffels VM, Schweickert A, Blum M, Franco D, Moorman AF 2001 Pitx2 expression defines a left cardiac lineage of cells: evidence for atrial and ventricular molecular isomerism in the iv/iv mice. Dev Biol (231):252-64
 [ PMID:10021341] Campione M, Steinbeisser H, Schweickert A, Deissler K, van Bebber F, Lowe LA, Nowotschin S, Viebahn C, Haffter P, Kuehn MR, Blum M 1999 The homeobox gene Pitx2: mediator of asymmetric left-right signaling in vertebrate heart and gut looping. Development (126):1225-34
Links:MGI:3530358 same experiment
 EMAGE:4092 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI