EMAGE mouse gene expression spatial database

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EMAGE:410

Fmn1 formin 1 ( MGI:101815)
TS17 (35 Somite no.)
in situ hybridisation

Data Images


Figure 1A of Zuniga et al., 1999 [PMID:9834182] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Image is of a forelimb bud. Arrowhead corresponds to posterior-distal mesenchymal cells.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

410_wholemount_strong_3D_1.wlz

410_wholemount_weak_3D_1.wlz

410_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:410_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb bud mesenchyme

strong

graded

Expression is highest in posterior-distal mesenchymal cells (arrowhead) and decreases towards the anterior in the distal mesenchyme.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:1314717
Entity Detected:	Fmn1, formin 1 ( MGI:101815)
Sequence:	sense strand is shown >MGI:1314717 GCCTACGAAAAGAAGAACAAAGTCAAAAAGATCATCAAGTTATTGGATGGAAAGCGATCTCAAACTGTGG GAATCTTGATATCTAGTTTACATCTAGAAATGAAAGATATTCAACAGGCCATATTTACTGTGGATGACTC CGTGGTTGACCTGGAGACCTTAGCAGCCTTATATGAAAATCGAGCCCAGGAGGATGAACTGACTAAAATA AGAAAGTACTATGAGACATCCAAAGAAGAAGACTTGAAACTGCTGGACAAACCTGAACAATTTTTGCATG AGTTAGCCCAGATTCCCAATTTTGCCGAACGTGCCCAGTGCATAATCTTCAGGGCTGTATTTTCTGAGGG TATCACATCCTTACATCGAAAAGTAGAGATTGTCACACGGGCCTCGAAGGGCTTGTTGCACATGAAGAGT GTGAAGGATATCTTAGCTCTCATTCTGGCTTTTGGAAACTACATGAATGGAGGAAACAGGACGCGAGGGC AAGCAGACGGATATAGTTTAGAAATTCTGCCCAAACTCAAAGACGTCAAAAGTCGGGACAATGGGATGAA TCTGGTGGACTATGTTGTGAAGTACTACCTGCGATACTATGATCAGTGCAAACATCATGACCAAGAAGCA AGTTGCAGAGGAAAGGATTTATTCAGCTTATACTTCCACATTGCTGTTCATCCCCAAAGGAAGTCAGGAC TGGAACTCAAGCAGGAAGCTGGAACAGACAAGAGTGTTTTCCCGCTGCCTGAACCACAGGATTTCTTCCT GGCCTCTCAAGTCAAGTTTGAAGACCTCCTAAAGGATTTGAGGAAGCTGAAGCGTCAACTAGAAGCAAGT GAGCAACAGATGAAGCTGGTGTGTAAGGAGTCCCCAAGGGAGTACCTGCAGCCTTTCAAGGACAAACTGG AGGAGTTCTTCAAGAAAGCCAAAAAAGAGCACAAGATGGAAGAAAGTCACTTGGAGAATGCACAGAAAAG TTTTGAAACAACAGTGGGATATTTTGGAATGAAGCCAAAGACTGGAGAGAAGGAGGTCACCCCCAGCTAT GTGTTTATGGTGTGGTTTGAGTTCTGCAGTGACTTCAAGACCATTTGGAAGCGGGAGAGTAAGAACATAT CTAAAGAAAGATTGAAAATGGCTCAGGCATCCGTCAGCAAACTGACATCAGAGAAGAAAGTGGAGACAAA GAAAATCAATCCCACCGCTAGTCTGAAAGAAAGACTGCGTCAGAAGGAAGCCAGCGTGGCCACCAACTAA GATGAAAACAGAAGGAAGTTACAATATGAAAGACAGCACCTCACTGCTCCTGCAGACACAAGGGCCACCG GGGAGTTCCTGAAAGACTAGTTAGGGACATTTGCTCATCTCCTTCTGAGGCCATCTGCAGAAAGTCCCCT GTGCCTTCTTTGGAAAGTCTCTTGTCAATCCACAAGAACAATGCAAAGTTATGTTTGGGAACACTACAGG AATATGTAATTGTTTCTTGCAGAAGTCTTAAGTCCACCCAATAATAAGATCAGAAGATATTTAATTCTTT TCACATTTTATTTATTTAAAGTGGAAATTCTAAGCCTTCCAGTATATACCCTTGTCATGAGAAAAGAGGT ACATGGTGATCCCCAAGAGAACTCAAGCCTTATTCCTCACACACT
	nt 3319 - nt 4973 of X53599.1
Notes:	The Formin probe used in this study by Zuniga & Zeller, 1999 [PMID:9834182] is defined as "being complementary to the 3' part (1.7 kb StuI-EcoRI probe, Woychik et al., 1990 [PMID:2392150] ) ". This probe is not mentioned by Woychik et al., however, there is a single StuI restriction site in Woychik's GenBank entry (X53599), 1654 nt from the 3' end.
Chemistry:	RNA
Strand:	antisense

Specimen

Organism:	mouse
Strain:	(C57BL/6 x SJL) F1
Age:	35 Somite no.
Theiler Stage:	TS17
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Zuniga & Zeller, 1999 [PMID:9834182] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:9834182] Zuniga A, Zeller R 1999 Gli3 (Xt) and formin (ld) participate in the positioning of the polarising region and control of posterior limb-bud identity. Development (126):13-21
	[ doi:10.1038/346850a0] [ PMID:2392150] Woychik RP, Maas RL, Zeller R, Vogt TF, Leder P 1990 'Formins': proteins deduced from the alternative transcripts of the limb deformity gene. Nature (346):850-3
Links:	MGI:1314731 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source