EMAGE mouse gene expression spatial database

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EMAGE:4111

Daam2 dishevelled associated activator of morphogenesis 2 ( MGI:1923691)
TS15 (9.5 dpc)
in situ hybridisation

Data Images


Figure 3F. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2004.06.001] Gene Expr Patterns 5: 97-105, Nakaya MA; Habas R; Biris K; Dunty WC Jr; Kato Y; He X; Yamaguchi TP, Identification and comparative expression analyses of Daam genes in mouse and Xenopus. Copyright 2004.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Note: fl, forelimb; li, liver; s, somite. Scale bar = 500 microns. Arrow = mDaam2 expression resolved into a strong spot of expression in the chordoneural hinge at the posterior-most end of the notochord

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4111_wholemount_strong_3D_1.wlz

4111_wholemount_moderate_3D_1.wlz

4111_wholemount_possible_3D_1.wlz

4111_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4111_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trunk somite

detected

regional

Expression in dermomyotomes.

notochord

strong

regional

Expression is at the chordoneural hinge at the posterior-most end of the notochord.

rhombomere 05

strong

rhombomere 03

weak

1st branchial arch mandibular component mesenchyme

detected

2nd branchial arch

detected

regional

Expression is posterior.

3rd branchial arch

detected

visceral organ system

detected

regional

Expression is in the liver.

forelimb bud mesenchyme

detected

single cell

trunk mesenchyme

detected

regional

Expression is in the thickening of the lateral mesoderm wall that will become the hindlimb.

future midbrain

not detected

homogeneous

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:3525598
Entity Detected:	Daam2, dishevelled associated activator of morphogenesis 2 ( MGI:1923691)
Notes:	The Daam2 probe used in this study by Nakaya et al., 2004 [PMID:15533824] is indicated as being synthesized "using T3 polymerase from the XhoI-cut EST BF721747 (289b.p.) or T7 polymerase from the SalI-cut EST BF783793 (691b.p.). Editors Note: "EST BF721747" = IMAGE clone 3971136 and "EST BF783793" = IMAGE clone 4237922. The probes are longer than is suggested by the authors as both IMAGE clone inserts are longer than the EST reads. It is not specified which of these two probes was used in this particular assay.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	9.5 dpc
Theiler Stage:	TS15
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:

4% paraformaldehyde

General Information

Authors:	Nakaya MA; Habas R; Biris K; Dunty WC Jr; Kato Y; He X; Yamaguchi TP, 2004 [PMID:15533824] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/j.modgep.2004.06.001] [ PMID:15533824] Nakaya MA, Habas R, Biris K, Dunty WC, Kato Y, He X, Yamaguchi TP 2004 Identification and comparative expression analyses of Daam genes in mouse and Xenopus. Gene Expr Patterns (5):97-105
Links:	MGI:3525572 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source