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EMAGE:4120

Tgfb3 transforming growth factor, beta 3 ( MGI:98727)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4120 EMAGE:4120 EMAGE:4120

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
The apparent staining in this specimen may be an artifact due to reagent trapping / specimen storage.
Expression Pattern Description
Spatial Annotation:
EMAGE:4120Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4120_wholemount_possible_3D_1.wlz
4120_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4120_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
hindbrain
strong strong
midbrain
moderate moderate
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:TGFB3_probeA
Entity Detected:Tgfb3, transforming growth factor, beta 3 ( MGI:98727)
Sequence:sense strand is shown

>TGFB3_probeA
TCCGGAGAATACACCCTGACCCGGAAGGTGTCTAGCCAAATATCCAAGGGGAAATGCGGCCAAGAAGGAA
GGCAGGAGGAGGGGCCCCCAGGGAATACATGAGAGAACCAATTCTGACCTCTGCTTTTGAGTCCAGCATC
TCTAAGGGACCTGAGATAGCTAGTTGCTGGCCCAGAAACTAATTACATGATCTGTGATGACTCATGATGC
TGATCGAGGTGAAAAGACAGTTGCAGAAAGAACGGATTGTCCTTAATTCCAGGCAGTGCAAGATATGATT
CCAGGGGACTTTGGCTTGGTAAACTGAAGAAAAATGCTTGGCCGCCTCTAACGATATCCCAATTCCCTAG
AGCAGATGTACTGCAGTGAGCAAAGCTGACCTCAGACCTGAAATGCCTTCCAGTCTGGCCTTCACCTGAC
TGACCACTCTACCCTTCATCTTTTGTAGGGTAGCCCGAGGCCCATTGCCACTCACAATGTCCACTCGCTA
TCCGTTCTCTACCCCACCACCTCTG
nt 86946123 - nt 86946637 of NC_000078.4
Notes:Primers used to PCR generate the probe template from mouse genomic DNA: Forward 5' AATTAACCCTCACTAAAGGCAGAGGTGGTGGGGTAGAGA (T3 sense) Reverse 5' TAATACGACTCACTATAGGTCCGGAGAATACACCCTGAC (T7 antisense)
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Jacqueline Ramsay, David R FitzPatrick
Principal investigator:David R FitzPatrick, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Submitted by:Jacqueline Ramsay, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE