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EMAGE:4125

Mbnl2 muscleblind-like 2 ( MGI:2145597)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4125
Figure 2A. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S1567-133X(03)00064-4] Gene Expr Patterns 3: 459-62, Kanadia RN; Urbinati CR; Crusselle VJ; Luo D; Lee YJ; Harrison JK; Oh SP; Swanson MS, Developmental expression of mouse muscleblind genes Mbnl1, Mbnl2 and Mbnl3. Copyright 2003.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: Fl (forelimb bud), BI (first branchial arch), He (heart), Nt (neural tube).
Expression Pattern Description
Spatial Annotation:
EMAGE:4125Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4125_wholemount_strong_3D_1.wlz
4125_wholemount_moderate_3D_1.wlz
4125_wholemount_weak_3D_1.wlz
4125_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4125_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
1st branchial arch mandibular component
strong strong
1st branchial arch maxillary component
strong strong
future brain
strong strong
heart
detected detected
forelimb bud
detected detected
otocyst
detected detected
eye
detected detected
Expression is in the optic eminence.
nose
detected detected
regionalExpression is in the frontonasal prominence.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2683159
Entity Detected:Mbnl2, muscleblind-like 2 ( MGI:2145597)
Notes:The Mbnl2 probe template used in this study by Kanadia et al., 2003 [PMID:12915312] was "isolated from brain...and subcloned into pGEM-T Easy (Promega)....To generate antisense RNA probes...the Mbnl2 [plasmid was] linearized with NcoI and transcribed with SP6 RNA polymerase".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Kanadia RN; Urbinati CR; Crusselle VJ; Luo D; Lee YJ; Harrison JK; Oh SP; Swanson MS, 2003 [PMID:12915312] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S1567-133X(03)00064-4] [ PMID:12915312] Kanadia RN, Urbinati CR, Crusselle VJ, Luo D, Lee YJ, Harrison JK, Oh SP, Swanson MS 2003 Developmental expression of mouse muscleblind genes Mbnl1, Mbnl2 and Mbnl3. Gene Expr Patterns (3):459-62
Links:MGI:2683242 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI