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EMAGE:4129

Etnk2 ethanolamine kinase 2 ( MGI:2443760)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4129
Figure 3A (right). Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2003.09.010] Gene Expr Patterns 4: 135-40, Hurley TM; McClive PJ; Sarraj MA; Sinclair AH, Eki2 is upregulated specifically in the testis during mouse sex determination. Copyright 2004.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4129Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4129_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4129_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
not detected not detected
homogeneous
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3033235
Entity Detected:Etnk2, ethanolamine kinase 2 ( MGI:2443760)
Notes:The Etnk2 probe used in this study by Hurley et al., 2004 [PMID:15161093] was generated as follows: "Eki2 [Etnk2] (Genbank entry AY212244) was identified as a differential transcript as part of a suppression subtraction hybridisation screen using PCR select (clontech), on 12.5 dpc urogenital ridges. The Soares mouse urogenital ridge NMUR library was then differentially screened with the resulting PCR fragments. Antisense and sense riboprobes were synthesised using T7 and T3 RNA polymerases, respectively.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:Swiss
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Hurley TM; McClive PJ; Sarraj MA; Sinclair AH, 2004 [PMID:15161093] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.modgep.2003.09.010] [ PMID:15161093] Hurley TM, McClive PJ, Sarraj MA, Sinclair AH 2004 Eki2 is upregulated specifically in the testis during mouse sex determination. Gene Expr Patterns (4):135-40
Links:MGI:3037004 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI