Type: | in situ hybridisation probe |
Identifier: | MGI:3033235 |
Entity Detected: | Etnk2, ethanolamine kinase 2 ( MGI:2443760) |
Notes: | The Etnk2 probe used in this study by Hurley et al., 2004 [PMID:15161093] was generated as follows: "Eki2 [Etnk2] (Genbank entry AY212244) was identified as a differential transcript as part of a suppression subtraction hybridisation screen using PCR select (clontech), on 12.5 dpc urogenital ridges. The Soares mouse urogenital ridge NMUR library was then differentially screened with the resulting PCR fragments. Antisense and sense riboprobes were synthesised using T7 and T3 RNA polymerases, respectively. |
Chemistry: | RNA |
Strand: | antisense |